摘要
目的研究转录因子Ets-1和基质金属蛋白酶-9(MMP-9)在胎膜早破中的表达和作用。方法胎膜早破(PROM)患者67例,胎膜未破裂组70例,分娩后取破口处胎膜组织,采用免疫组织化学法染色,并使用病理影像多媒体图文操作系统对Ets-1和MMP-9阳性表达物的面积积分光密度(AIOD)进行半定量分析。结果①Ets-1在胎膜滋养层细胞核及胞浆中均有表达,且细胞核表达明显;MMP-9在胎膜滋养层细胞中的表达,主要位于细胞浆中;②Ets-1在胎膜早破组(0.0373±0.0119)中高表达,与胎膜未破裂组(0.0062±0.0089)比较,差异有高度统计学意义(P<0.001);③MMP-9在胎膜早破组(0.0928±0.0453)中高表达,与胎膜未破裂组(0.0345±0.039)比较,差异有高度统计学意义(P<0.001)。结论Ets-1和MMP-9在人类胎膜上均有表达,且在胎膜早破中高表达且具显著相关性,表明Ets-1可能通过上调MMP-9的基因表达量使胎膜细胞外基质(ECM)的降解加速致胎膜紧张度降低,引发胎膜早破。
Objective To study the expression and effect of the transcription factor Ets - 1 and matrix metalloproteinases - 9 (MMP - 9) in premature ruptured membranes. Methods Sample of chorioamniotic membranes near the rupture site were obtained from 67 premature rupture of membranes (PROM) and 70 non- PROM patients after labor. Semiquantitative analysis of the area integrated optical density (AIOD) of the positive expression of Ets - 1 and MMP - 9 were determined by immunohistoehemistry and pathologic image multimedia operation system. Results ① Ets- 1 could be detected both in cytoplasm and nucleus of trophoblastic cells and the expression was more stronger in nucleus. MMP - 9 could only be detected in nucleus of trophoblastic cells. ② The AIOD values of Ets- 1 in PROM group (0. 037 3±0. 011 9) was significantly higher than that in non- PROM group (0. 006 2± 0. 008 9, P〈0. 001). ③ The AIOD values of MMP- 9 in PROM group (0. 092 8±0. 045 3) was higher compared with that of non- PROM group (0. 034 5±0. 039, P〈0. 001). Conclusions Ets- 1 and MMP- 9 were expressed in human fetal membranes and their expression was up - regulated in PROM group. Ets - 1 may participate and play an important role in PROM through activating the transcription of matrix- degrading MMP- 9.
出处
《中国妇产科临床杂志》
2009年第4期268-270,共3页
Chinese Journal of Clinical Obstetrics and Gynecology
关键词
转录因子ETS-1
基质金属蛋白酶-9
细胞外基质
胎膜早破
transcription factors Ets - 1
matrix metalloproteinases - 9
extra cellular matrix
premature rupture of the membranes