摘要
Background and Objective Lung cancer is the leading cause of cancer death in both men and women. Tumor metastasis is an essential aspect of lung cancer progression. Nm23-H1 is
Background and Objective of cancer death in both men Lung cancer is the leading cause and women. Tumor metastasis is an essential aspect of lung cancer progression. Nm23-H1 is a metastasis suppressor gene involved many carcinomas, in addition, it correlated to cell proliferation, differentiation, cell signal transduction individual development and so on. However, the molecular mechanism by which nm23-H1 suppresses the metastasis is still unclear. Our previous studies in which Prof. Qinghua Zhou was the principal investigator demonstrated that transfection of exogenous nm23-H1 into the L9981 increased cell apoptosis and decreased metastatic ability. Furthermore, we found that the expression of β-Catenin, E-cadherin and TIMP-1 were significantly increased while the expression of Ras, MMP-2, CD44v6, and VEGFC dramatically decreased in L9981 cell line transfected with exogenous nm23-H1. We also found that G0/G1 phase cells markedly increased after transfection of exogenous nm23-H1. Here, for further confirm and discuss the correlation between tumor metastasis and nm23-H1, meanwhile as an extensive study and research, we compared the gene expression profile and biological behaviors of human large cell lung cancer cell line NL9980 by nm23-H1 gene silencing with that of negative control cells to comprehensively investigate nm23-H1-mediated changes in gene expression of NL9980 cells and further analyze how nm23-H1 as a key tumor suppressor gene regulate tumor metastasis/metastasis suppressor balance, which could lead to cascading response.
出处
《中国肺癌杂志》
CAS
2009年第6期519-520,共2页
Chinese Journal of Lung Cancer
基金
supported by the following grants to Qinghua ZHOU:The Key Project of National Natural Science Foundation of China (304303 00)
The Major Project of Tianjin Sci-Tech Support Programme (07SYSYS F05000)
The Key Project of Tianjin Sci-Tech Support Programme (06YFS ZSF05300)
The Building Project of Tianjin Sci-Tech Innovation Platform (07SYSYJC27900)
863 National Major Projects (2006AA02A401)
National Key Basic Research and Development Plan (973 Plan) program (20 07CBS914800)
关键词
肺癌
癌细胞
扩散
医学
治疗
Nm23-H1 gene
siRNA
RNA interference
Lungcancer
NL9980 cell
Tumor metastasis suppressor gene
