摘要
目的构建腺病毒五邻体基因重组表达质粒。方法通过PCR方法扩增获得腺病毒五邻体基因的完整序列,将此片段定向克隆到pQE30载体的多克隆位点区,筛选重组阳性菌株,提取重组质粒,利用酶切鉴定方法与核酸序列测定验证重组质粒构建的正确性。结果用PCR法获得了人腺病毒的五邻体基因,将该基因正确地重组到表达质粒pQE30中,构建出腺病毒五邻体基因重组表达质粒pQE30-penton。结论构建腺病毒五邻体基因重组表达质粒pQE30-penton。
Objective To construct human adenovirus （Ad） penton plasmid and express the penton gene. Methods The target DNA fragments of penton gene of adenovirus was obtained by PCR amplification and inserted into vector plasmid pQE30. The constructe＇d recombinant plasmid was identified by endonuclease digestion and DNA sequencing. Results The penton gene of adenovirus was obtained by PCR amplification. The penton gene was inserted into expressing plasmid pQE30 and the recombinant plasmid pQE30-penton was constructed. Conclusion The Ad penton recombinant plasmid pQE30-penton was constructed successfully.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2009年第3期218-221,共4页
Journal of Harbin Medical University
基金
国家自然科学基金项目(30771909)
黑龙江省自然科学基金重点项目(ZJY0701)
教育部博士点专项基金(20070226007)
关键词
腺病毒
五邻体
重组质粒
序列分析
adenovirus
penton
recombinant plasmid
sequence analysis
