摘要
目的:探讨大鼠皮肤切创愈合过程中E-选择素(E-selectin)及细胞间粘附分子-1(Intercellular adhesion molecule-1,ICAM-1)表达变化规律及法医学意义。方法:建立模型后,应用免疫组化方法,观察大鼠生前皮肤切创后不同时间点E-选择素及ICAM-1的表达变化,并用图像分析系统对图像分析。结果:正常组无E-选择素表达,切创后1hE-选择素在损伤周围血管内皮细胞有阳性表达,伤后1d达峰值,以后开始下降,持续至伤后7d,且E-选择素随时间呈规律性变化。ICAM-1最早在伤后1h,最迟在伤后3d在表皮层中呈阳性表达。此外,ICAM-1还在炎症细胞、成纤维细胞及损伤周围血管内皮细胞有阳性表达,且随损伤时间呈规律性变化。E-选择素及ICAM-1在死后伤中均为阴性表达。结论:E-选择素及ICAM-1可作为法医学损伤时间的参考指标。
Objective：To investigate the expression regulation of E-selectin and intercellular adhesion molecule-1 （ICAM-1） in the heading process of incised wounds of rat skin and its significance in forensic medicine. Methods： After establishment of model, a study on the expression of E-selectin and ICAM-1 in vital rats skin incised wounds at different times were performed by immunohistochemistry technique, and evaluated with image analysis technique and statistic system. Results：There was no expre -ssion of E-selectin in the skin of normal control group. Positive expression of E-selectin could be observed on epidermis cells surface around injury lh after injury. The expression level of E-selectin reached its peak on day 1 and then started to decline,lasted till day 7.The ratios of E-selectin positive vascular endothelial cells changed regularly according to different time after wound.ln the vital skin incisions,while positive stain of ICAM-1 stain was observed on vascular endothelium as early as 1 h after incision and lasted till 3d.In addition, the expression of ICAM-1 was also found in the inflammatory cells and fibroblasts and on vascular endothelium surface around injury,the lasting period varied from group to group There were no positive expression of E-selectin and ICAM-1 in the postmortem incision group. Conclusion：E-selectin and ICAM-1 are considered potentially useful markers for timing of wound age determination in forensic practice.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第7期908-911,共4页
Journal of Chongqing Medical University
