摘要
目的:研究石见穿全草的水溶性化学成分及进行含量分析。方法:利用水提、有机溶剂萃取及采用葡聚糖凝胶LH-20柱进行分离纯化,采用HPLC进行定量分析;结果:从石见穿水提取物中分得丹参素和迷迭香酸。HPLC色谱条件下,丹参素进样量在1.48～7.40μg范围内与峰面积积分值呈良好线性关系(r=0.999 9),丹参素加样回收率(按丹参素钠计算)平均值为102.8%,RSD=1.04%;原儿茶醛进样量在0.05～0.26μg范围内与峰面积积分值呈良好线性关系(r=0.999 9),原儿茶醛加样回收率平均值为101.5%,RSD=0.72%。结论:为石见穿的质量控制提供了可靠的检测方法。丹参素为首次从该植物中分离得到。
Objective： To study and determine the Water-soluble substances of Salvia chinensis. Methods： The constituents of the n-Butanol-soluble portion of the water extractive were isolated and purified by means of Sephadex LH- 20 column chromatographic methods. HPLC was used to determine the contents. Results： Two compounds were isolated and identified as danshensu and rosmarinic acid. The linear range of danshensu was 1.48 - 7.40 μg. The average recovery was 102.8% and RSD was 1.04% ; The linear range of protocatechualdehyde was 0.05 - 0.26μg. The average recovery was 101.5 % and RSD was 0.72%. Conclusion： The method can provide useful references to quality control of Herba Salvia chinensis. Danshensu was firstly isolated from Herba Salvia chinensis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第7期1-3,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国际科技合作重点项目计划(2005DFA31000)
关键词
石见穿
丹参素
迷迭香酸
原儿茶醛
含量测定
Herba Salvia chinensis
danshensu
rosmarinic acid
protocatechualdehyde
assaying
