摘要
以石斛叶片为材料,建立了一套适用于石斛蛋白质组分析的双向电泳体系,探讨了石斛叶片蛋白的提取、蛋白定量以及双向电泳的一些关键技术。结果表明,通过三氯乙酸(TCA)法提取、高压等电聚焦和两步平衡等方法可以获得稳定、清晰的双向电泳图谱,获得了可分辨蛋白质斑点数约613个。
A 2-D （two-dimensional electrophoresis） protocol suitable for protein analysis in dendrobium leaf was set up. The protein extraction and protein excretion, as well as some key technologies of 2-D electrophoresis were studied. The results showed that stable and clear 2D maps with about 613 protein spots could be obtained through the methods of extraction with trichloroacetic acid, isoelectric focusing under high-voltage and two-step equilibrium.
出处
《浙江农业学报》
CSCD
北大核心
2009年第3期230-233,共4页
Acta Agriculturae Zhejiangensis
基金
国家自然科学基金(30670199
30770185)
浙江省自然科学基金(305692
301406)
浙江省科技计划(2006C32016)
杭州市属高校重点实验室科技创新计划(20080432T06)
关键词
双向电泳
蛋白质组
石斛
two-dimensional electrophoresis
proteome
Dendrobium orchids
