摘要
从对百草枯具有高度抗性的硝化还原假单胞菌(Pseudomonas nitroreducens)菌株SPQ03中分离了PnSoxR和PnSoxS调控子。ScanProsite程序分析结果表明,SoxR氨基端含有一个MerR家族特有的DNA结合域,羧基端含有4个半胱氨酸残基,是MerR家族成员之一;SoxS是AraC家族成员,含有2个典型的AraC-HTH保守域;PnSoxR所编码的氨基酸与大肠杆菌SoxR的一致性为98%,而与同属的铜绿假单胞菌(Pseudomonas aeruginos)SoxR的一致性仅为56%,PnSoxR与大肠杆菌SoxS的一致性为100%,铜绿假单胞菌中没有发现SoxR基因存在。将PnSoxR和PnSoxS分别转化E.coliBL21,发现PnSoxS能赋予BL21百草枯(Paraquat)抗性。
Transcription factor PnSoxR and PnSoxS were cloned from Pseudomonas nitroreducens SPQ03 which possessed strong resistance to paraquat. Based on SoxR and SoxS proteins sequences, the ScanProsite procedure analysis indicated that SoxR protein which contained a HTH-DNA binding motif in N-end and four cysteines in the C-end was one of MerR family members. There were two typical AraC-HTH conservative domains in the C-terminal of SoxS protein, and SoxS belonged to the AraC family member. The protein sequences of PnSoxR and PnSoxS shared high identity with E. coli SoxRS （SoxR 98%, SoxS 100% ） , and 56% with Pseudomonas aeruginos. The engineering strain carried PnSoxR or PnSoxS gene was transformed into E. coli BL 21, and the result showed that PnSoxS engineering strain increased resistance to paraquat （PQ）.
出处
《江苏农业学报》
CSCD
北大核心
2009年第3期524-528,共5页
Jiangsu Journal of Agricultural Sciences
基金
江苏省博士后科研资助计划项目(0701021B)
江苏省农业科学院博士后科研资助计划项目(005036510701)
国家转基因专项项目(20082X200805-001)
