摘要
背景:透明质酸具有与受体特异结合的特性,将其与阿霉素联合制成纳米粒,可以充分发挥药物靶向作用,降低药物的毒副作用。目的:探讨阿霉素-透明质酸纳米颗粒于体外靶向杀伤口腔鳞状细胞癌的作用。设计、时间及地点:体外对比观察实验,于2008-01/07在中国海洋大学药理学实验室完成。材料:人舌癌T8113细胞株由上海第九人民医院陈万涛教授惠赠,阿霉素-透明质酸纳米粒由中国海洋大学海洋生命学院惠赠。方法:在体外培养的口腔鳞状细胞癌细胞中分别加入阿霉素质量浓度为0.5,1.0,5.0,10.0mg/L的阿霉素-透明质酸纳米颗粒,利用体外细胞毒实验四甲基偶氮唑蓝法检测阿霉素-透明质酸纳米颗粒对肿瘤细胞的靶向杀伤作用;阿霉素质量浓度为5.0,10.0mg/L时分别作用0,6,12,24,48h后,利用流式细胞仪检测其对肿瘤细胞凋亡的作影响。主要观察指标:①细胞生存率。②细胞凋亡率。结果:24,48h细胞毒试验阿霉素-透明质酸纳米颗粒对肿瘤细胞的杀伤作用优于游离阿霉素(t=5.78～42.05,P<0.01)。相同浓度阿霉素-透明质酸纳米颗粒细胞凋亡百分率随时间增加而升高(F=4200.40,4775.36,P<0.01),相同作用时间凋亡率随浓度增加而升高(t=12.06～20.08,P<0.05)。结论:阿霉素-透明质酸纳米颗粒可特异性识别肿瘤细胞,并实现其杀伤作用,并随时间延长增加杀伤作用。
BACKGROUND： Adriamycin （ADM） can specifically conjugate with receptor. In particular, ADM nanoparticles play a target role in decreasing the cytotoxicity. OBJECTIVE： To investigate the targeting effect of adriamycin nanoparticles conjugated with hyaluronic acid （ADM-HA-SSL） on oral squarnous carcinomas cells in vitro. DESIGN, TIME AND SETTING： An in vitro contrast observation was performed in College of Marine Life Science, Ocean University of China from January to July 2008. MATERIALS： Oral squamous carcinomas cells were sincerely presented by Professor Chen from the Ninth People＇s Hospital of Shanghai; ADM-HA-SSL （drug loading 156 mg/L） was sincerely presented by Professor Liu from College of Marine Life Science, Ocean University of China. METHODS： Oral squamous carcinomas cells were cultured with 0.5, 1.0, 5.0, and 10.0 mg/L ADM-HA-SSL. MTT assay was used to detect the targeted cytotoxicity of ADM-HA-SSL against oral squamous cell carcinomas. With the concentrations of 5.0 and 10.0 mg/L, cell apoptosis was ascertained by cell flow cytometry after 6, 12, 24 and 48 hours. MAIN OUTCOME MEASURES： Cell survival rate and apoptosis rate. RESULTS： At 24 and 48 hours after induction, cytotoxicity assay revealed that the effect of ADM-HA-SSL was superior to that of free ADM （t=5.78 42.05, P 〈 0.01）. The results of flow cytometry showed that the apoptosis rate was enhanced with the increase of the time （F=4 200.40, 4 775.36, P 〈 0.01 ）, and the rate was also increased at the same time point with the increase of concentration （t=12.06-20.08, P 〈 0.05）. CONCLUSION： ADM-HA-SSL can specifically recognize oral squamous carcinomas cells and deliver adriarnycin into the cells. And the effect is enhanced by the time prolonging.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第25期4985-4988,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
the Foundation of Shandong Province for Young Scientists, No 2006BS03044~~
