摘要
背景:引起高分子材料相关感染的主要原因是材料表面形成了生物被膜,因此要预防此类感染不仅需要材料对表面接触的浮游菌有良好的抑制和杀灭效果,更需要抑制致病菌在表面形成生物被膜。目的:观察抗感染聚乙烯材料的抗菌效果以及在菌液中对材料表面生物被膜形成的抑制作用。设计、时间及地点:体外对比观察实验,于2007-01/2008-01在中科院理化所工程塑料国家工程研究中心完成。材料:有机抗菌剂为2,4,4’-三氯-2’-羟基二苯醚;无机抗菌剂为玻璃基银系抗菌剂;低密聚乙烯。方法:低密度聚乙烯与有机和无机两种抗菌剂混合后先挤出造粒,然后注塑成片。主要观察指标:①用活菌平板计数法测定材料的抗菌性能。②平板超声波法检测材料表面细菌密度及判断生物被膜的形成情况。结果:①两种改性抗菌材料对大肠杆菌和金黄色葡萄球菌都有良好的抗菌效果,抗菌率达到99.9%。②添加有机抗菌剂的试样可以在生物被膜成熟前显著杀灭样品表面的细菌,阻止细菌在其表面黏附和繁殖,从而抑制生物被膜形成。空白试样和无机抗菌试样表面均有大量细菌黏附。结论:有机抗感染聚乙烯材料对大肠杆菌和金黄色葡萄球菌有良好的抑菌作用,可抑制细菌在材料表面形成生物被膜。
BACKGROUND： Infection of medical polymers starts with the initial adhesion of infectious organisms that subsequently grow to form a biofilm. Thus polymer materials which possess inhibiting effect to biofilm formation can reduce infection rate. OBJECTIVE： To evaluate the antimicrobial efficacy of modified polyethylene samples and the inhibitory effect of antimicrobial agent on biofilm formation on the polyethylene surface. DESIGN, TIME AND SETTING： A controlled observation experiment in vitro was performed at the State Engineering Research Center of Engineering Plastic, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences （Beijing, China） between January 2007 and January 2008. MATERIALS： Organic antibacterial agent was triclosan. Inorganic antibacterial agent was ionpure. Low-density polyethylene （LDPE） was used for this study. METHODS： LDPE was respectively blended with organic antibacterial agent and inorganic antibacterial agent, and the LDPE slice was prepared by injection molding process. MAIN OUTCOME MEASURES： The antimicrobial efficacy of anti-infective polyethylene was determined by the plate counting method. The ultrasonic-plate method was introduced to observe bacterial density and biofilm formatiom. RESULTS： Both of modified LDPE samples exhibited excellent antimicrobial performance against S. aureus and E. coil, resulting in a antibacterial rate of 99.9%. The adhesion and propagation of bacteria were evidently reduced by organic antibacterial agent added into LDPE, thereby the formation of biofilm was prevented. The results showed that a large amount of bacteria adhered on the surfaces of LDPE added inorganic antibacterial agent and untreated LDPE. CONCLUSION： Anti-infective polyethylene added organic antibacterial agent possesses excellent antimicrobial performance against S. aureus and E. coil, and it can inhibit the formation of biofilm on the surfaces.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第25期4871-4874,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
中国科学院知识创新工程项目资助(KGCX2-YW-608)~~
