摘要
为了改变传统的将两栖类动物处死后再从肌肉提取DNA的取样方法,采用非伤害性取样方法分别采集了虎纹蛙的血液和趾提取DNA,并与从其肌肉中提取的DNA进行了比较,结果显示:使用血液和趾提取的DNA产量与肌肉样本的DNA产量相当;随后,分别对3种方法取样的样本进行了线粒体16S rRNA和核DNA的微卫星PCR分析,结果表明:3种取样方法所获得的扩增效果相当,均能满足虎纹蛙的保护遗传学研究.因此认为,用非伤害性取样技术替代传统的肌肉取样的方法在无尾两栖类的保护遗传学研究中是切实可行的.
In order to avoid executing amphibian to get DNA from their muscle in the traditional method, it was intended to use nondestructive sampling to extract DNA from blood and toes of tiger frogs respectively, and compared with the DNA which was extracted from the muscle. The results showed that the DNA from blood and toes samples was equally applicable with the muscle sample. Then PCR analysis was applied to mitochondrion 16S rRNA and the nuclear DNA simple sequence repeated of the three types of samples respectively. The results also showed that the amplification products in three types of sampling were equivalent, and all were appropriate for conservation genetics research.
出处
《浙江师范大学学报（自然科学版）》
CAS
2009年第3期322-325,共4页
Journal of Zhejiang Normal University:Natural Sciences
基金
浙江省自然科学基金资助项目(Y506234)
浙江省科技计划重点项目(2008C22057)
