摘要
目的建立稳定的肝动脉重建的小鼠原位肝移植模型。方法以“双袖套法”小鼠肝移植为基础，将带腹主动脉的供肝肝动脉与受体腹主动脉行端侧吻合。共完成小鼠原位肝移植70例，并观察术后24h、1周、1个月受体存活率，术后1个月肝功能及肝组织病理变化。同时设立假手术组对照。结果受体术后24h、1周、1个月存活率分别为94．3％、91．4％、85．7％。术后1个月肝功能ALT：（39．20±2．09）U／L；AST：（75．60±3．24）U／L。与对照组比较，差异无统计学意义（P〉0．05）。病理显示肝组织结构良好。结论该方法可稳定地建立肝动脉重建的小鼠原位肝移植模型。
Objective -To establish the arterialized liver transplantation model in mice. Methods On the basis of ＂two cuff＂ orthotopic liver transplantation in mice, graft aorta end-to-side anastomosis to recipient aorta was applied to reconstruct hepatic artery. Twenty-four h-, 1 week-, 1 month-postoperative survival rate, hepatic function, and pathological changes were observed. Sham operation group was set up. Results 94.3% ,91.4% and 85.7% of recipients survived after 48 h,one week and one month,respectively. The liver functioned normally after one month postoperatively. Pathologically, the hepatic tissue had good structure. Conclusion It is an ideal mothods to establish the arterialized orthotopic liver transplantation model in mice.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第7期931-932,I0004,F0003,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（30772055）
重庆市卫生局科研资助项目（06-2-017）
关键词
肝移植
肝动脉重建
模型
动物
Liver transplantation
Hepatic rearterialization
Model, animal
