摘要
目的:研究白藜芦醇(resveratrol,Res)影响人胃癌细胞SGC-7901增殖及凋亡的机制。方法:使用不同浓度Res处理SGC-7901细胞不同时间后,采用MTT法测定肿瘤细胞生长抑制率;FCM和TUNEL法检测分析细胞周期和细胞凋亡率;免疫细胞化学法检测细胞中bcl-2、bax、caspase-3和Fas蛋白的表达;RT-PCR检测Fas、bcl-2、bax、caspase-3 mRNA的表达。结果:Res对胃癌SGC-7901细胞的生长抑制作用随着Res作用时间的延长和浓度的增加而明显增强(P<0.01,P<0.05),同时能诱导其发生凋亡(P<0.01);Res(150μmol/L)能明显上调SGC-7901细胞中bax、caspase-3、Fas mRNA及其蛋白的表达,下调bcl-2 mRNA及其蛋白的表达。结论:Res抑制SGC-7901细胞的增殖、诱导细胞凋亡呈浓度、时间依赖性;这可能与Res上调肿瘤细胞Fas、bax和caspase-3的表达及下调bcl-2的表达有关。
Objective ： To investigate the effects of resveratrol （Res） on cell proliferation and apoptosis of gastric adenoeareinoma SGC-7901 ceils and their action mechanism. Methods： SGC-7901 ceils were treated with different concentrations of Res for various periods. The cell proliferation was examined by MTT assay. TUNEL method and flow cytometry were used to analyze cell cycle and detect apoptosis. Expressions of bcl-2, bax, caspase-3 and Fas proteins in gastric cancer cells were measured by immunohistochemistry. RT-PCR was used to detect the mRNA transcriptions of bel-2, bax, caspase-3 and Fas. Results： Res inhibited the growth of gastric adenocarcinoma SGC-7901 cells in time- and concentration-dependent manners （P 〈 0.01, P 〈 0.05 ）. Res induced apoptosis of SGC- 7901 cells （P 〈0.01 ）. Res（ 150 μmol/L） significantly up-regulated the mRNA and protein expressions of bax, caspase-3, and Fas and down-regulated gene transcription and protein expression of bcl-2. Conclusion： Res inhibited the growth and induced apoptosis of gastric adenocarcinoma SGC-7901 cells in time- and concentration-dependent manners. It was related with up-regulation of expression of Fas, bax, caspase-3 and down-regulation of expression of bcl-2.
出处
《肿瘤》
CAS
CSCD
北大核心
2009年第6期523-526,共4页
Tumor
