摘要
目的探讨实时荧光定量PCR检测金黄色葡萄球菌femA表达的方法。方法以BB270femA基因表达为标准,建立实时荧光定量PCR方法,对实验菌株femA表达进行相对定量。结果建立的实时荧光定量PCR方法所得结果标准曲线线性关系好,熔解峰单一。随MIC变化,金黄色葡萄球菌femA表达水平也随之变化。结论用实时荧光定量PCR方法研究金黄色葡萄球菌femA表达量结果可靠、敏感、重复性好。
Objective To establish the approach of real -time fluorescent quantitative PCR to detect femA gene in Staphylococcus aureus strains. Methods Real - time fluorescent quantitative PCR was performed to quantify the expression offemA gene of 15 clinical stains, as compared with BB270. Results The standard curve by real - time fluorescent quantitative PCR showed good linearity between the amount offemA RNA and cycle threshold and the melting curve had a single peak. The variation of expression level offemA was in accordance with MIC of strains. Conclusions The real - time fluorescent quantitative PCR in the detection offemA expression is accurate, sensitive and repeatable.
出处
《徐州医学院学报》
CAS
2009年第6期391-393,共3页
Acta Academiae Medicinae Xuzhou
