摘要
从黑龙江省某3个猪场采集疑似为日本脑炎病毒感染的病、死猪的脏器以及全血，进行病毒的细胞培养，蚀斑纯化，间接免疫荧光试验，电镜下观察病毒粒子的形态，及RT—PCR检测。结果显示，盲传三代后，出现明显的细胞病变（CPE＋～CPE＋＋＋）；荧光显微镜下细胞胞质内呈现不同强度的绿色荧光信号；电镜下的病毒粒子为40-60nm的球型粒子；PCR检测为日本脑炎病毒阳性。通过对3株分离株腑扩增序列比较，此3株分离株为基因Ⅲ型。
To study virus by affection and dying swine in Heilongjiang Province in 2008, virus strains were isolated by inoculating BHK-21, plaque purification, IFA, electron microscope and RT-PCR. The results showed that three strains were isolated from affection and dying swine causing cytopathogenic effect CPE（＋--＋＋＋） on cells after blind passage tert, All the 3 isolated which were identified to be Japanese encephalitis viruse were isolated from swine. The phylogenetic analysis showed that the new isolates belonged to genotype Ⅲ.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2009年第6期73-78,共6页
Journal of Northeast Agricultural University
基金
黑龙江省科技攻关计划重点项目(GB05B01-1)
