摘要
目的:通过长期的中等强度运动对大鼠金属硫蛋白(MT)含量的影响,来探讨MT对心肌的保护作用及其机制。方法:选用健康雄性SD大鼠30只,体重220～250 g,随机分为安静对照组(C组);中等强度(90 min)运动训练组(B组);一次性力竭运动组(A组);每组10只。B组大鼠进行为期8周的中等强度运动。测定大鼠心肌组织中的MT、组织中钙、丙二醛(MDA)、硫基(-SH)含量以及谷胱甘肽过氧化物酶(GSH-px)和Na+-K+-ATP酶的活性。结果:中等强度运动组较对照组大鼠心肌组织中MT的含量、GSH-px活性和Na+-K+-ATP酶的活性显著增高(P<0.05),MDA含量和总钙量显著降低(P<0.05);-SH含量没有显著性差异;但-SH含量有升高趋势。力竭运动组大鼠心肌组织中MT和-SH含量,GSH-px和Na+-K+-ATP酶的活性较对照组显著降低(P<0.05),MDA含量和总钙量显著升高(P<0.05)。结论:长期中等强度的运动可提高心肌的抗氧化能力,其机制可能与心肌中MT增加有关。
Objective： To discusses the protective effects of long-term, medium intensity exercise on heart. Methods： The 30 Sprague--Dawley rats were selected and randomly divided into 3 groups： group A was the exhaustive swimming exercise group, group B was the medium intensity group （90rain swimming exercise/d, eight weeks）, group C was a control group without exercise （control group）, with 10 rats in each group. The contents of MT, MDA,-- SH, the activity of GSH-px and Na^＋ -K＋ -ATP in myocardial of rats were measured. Results： Compared with control group, the MT contents obviously increased, the activity of GSH -- px and Na^＋ -- K^＋ -- ATP obviously increased, while the level of MDA and total of calcium significantly decreased in the rats of medium intensi＇cy exercise group （P〈0. 05 all）. The contents of MT, --SH notably reduced, the activity of GSH--px, and Na^＋ --K^＋ --ATP obviously decreased, while the levels of MDA and total of calcium obviously increased in the rats of exhaustive swimming exercise group （P〈0. 05 all）. Conclusion： Long-term medium intensity exercise raise the antioxidant defense in myocardial of rats. Its mechanism may be MT levels increase in cardiac.
出处
《心血管康复医学杂志》
CAS
2009年第3期214-216,259,共4页
Chinese Journal of Cardiovascular Rehabilitation Medicine
关键词
金属硫蛋白
运动
心肌
Metallothionein
Exercise
Myocardium
