摘要
目的:观察冠心Ⅱ号核心组成药物丹参、川芎、红花合煎剂(丹川红)对大鼠心肌缺血模型心肌细胞凋亡的影响。方法:缺血前0.5h给大鼠灌胃冠心Ⅱ号标准汤剂、丹川红或等容积生理盐水,采用结扎左冠状动脉前降支法建立大鼠急性心肌缺血模型。3h后收集大鼠心脏,应用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法(TUNEL)观察大鼠心肌缺血区的心肌细胞凋亡情况或24h后收集大鼠心脏,应用氯化三苯基四氮唑(TTC)组织化学染色法测量心肌梗死面积。结果:各给药组心肌梗死面积(Infarct Size)和心肌细胞凋亡指数(Apoptosis Index)显著低于模型对照组;其中,丹川红高剂量组与冠心Ⅱ号标准汤剂组、丹川红低剂量组相比,心梗面积和凋亡指数无统计学差异;丹川红低剂量组与冠心Ⅱ号标准汤剂组相比,结果有统计学差异。结论:由药物丹参、川芎、红花组成的丹川红合煎剂与原方冠心Ⅱ号疗效一致,都能通过抑制心肌细胞凋亡以减少心肌缺血损伤,起到保护心肌作用。
Objective：To observe the effects of Dan-Chuan-Hong decoction on myocardial apoptosis of acute myocardial ischemia in rats. Methods：Rats received Guan-Xin-Er-Hao,Dan-Chuan-Hong or normal saline at 30 minutes before ischemia. The model of acute myocardial infarction （AMI） in rats was set up by ligating the descending branch of left coronary artery. 3 hours after AMI, the hearts were isolated to detect the apoptosis of myocardial ischemia by TUNEL;24 hours after AMI ,the hearts were isolated to determine the area of myocardial infarction by quantitative histological assay （triphenyhetrazolium chloride,TTC stain）. Results：Comparing with model group,the myocardial infarct size and apoptosis index of all the treatment groups decreased. Comparing with Guan-Xin-Er-Hao （30 g/kg）or Dan-Chuan-Hong （ 10 g/kg）, the myocardial infarct size and apoptosis index of Dan-Chuan-Hong （20 g/kg）had no statistic difference. And there was statistic difference between Guan-Xin-Er-Hao （30 g/kg）and Dan-Chuan-Hong （10 g/kg）. Conclusion： Comparing with Guan-Xin-Er-Hao ,the decoction of Dan-Chuan-Hong has similar effect ,which could protect hearts from myocardial ischemia by inhibiting the myocardial apoptosis.
出处
《中药材》
CAS
CSCD
北大核心
2009年第5期725-728,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(30572339)
国家杰出青年基金(30325045)
关键词
丹川红
冠心Ⅱ号
心肌缺血
细胞凋亡
Dan-Chuan-Hong
Guan-Xin-Er-Hao
Myocardial ischemia
Apoptosis
