摘要
目的检测磷脂酰肌醇3激酶（P13K）、磷酸化蛋白激酶B（P-AKT）、蛋白激酶B（AKT）在宫颈癌中的表达，进一步探讨其与宫颈癌发生、发展的关系。方法应用蛋白免疫印迹技术检测66例宫颈标本（包括33例宫颈癌、23例宫颈上皮内瘤样病变、10例正常宫颈组织）P13K、AKT、P-AKT表达情况，并结合临床资料分析其临床意义。结果宫颈癌、宫颈上皮内瘤样病变、正常宫颈组织中P13K表达量分别为1．3880±0．0435、0．5330±0．0939、0．2427±0．0888，三者比较差异有统计学意义；P—AKT／AKT分别为5．8702±0．0543．5．0755±0．0888、3．8353±0．0056，三者比较差异无统计学意义。宫颈鳞癌与宫颈腺癌和腺鳞癌的P—AKT／AKT分别为6．7823±0．7745和0．7621±0．0709，宫颈鳞癌P—AKT／AKT表达明显高于宫颈腺癌和腺鳞癌，差异有统计学意义。结论P13K过表达与宫颈癌的发生有一定的关系；P-AKT／AKT高表达与宫颈癌的病理类型有关。
Objective To determine the expression of phosphatidylinositol 3- kinase （PI3K）, phosphorylated protein kinase B （P-AKT） and protein kinase B （AKT） in cervical cancer and explore the correlations with the genesis and development of cervical cancer. Methods Western blot was used to detect the expression of PI3K, P-AKT and AKT proteins in 66 cases of cervical tissues, including 33 cervical cancers, 23 cervical intraepithehal cancer, 10 normal cervical tissues, and analyzed the clinical significances according to clinical informations. Results In cervical cancers, cervical intraepithelial cancer and normal cervical tissues, the expression of PI3K protein were 1.3880 ± 0.0435, 0.5330 ±0.0939, 0.2427 ± 0.0888 and had significant differences among them, but there was no significant difference in the ratio of P-AKT and AKT, which were 5.8702 ± 0.0543, 5.0755±0.0888, 3.8353 ±0.0056. The ratios of P-AKT and AKT had association with histological type, the ratios in squamous cancer of the cervix and in adenocancer and denosquamous cancer were 6.7823± 0.7745 and 0.7621±0.0709, and the ratios were much higher in squamous cancer of the cervix than adenocancer and denosquamous cancer. Conclusions Overexpression of PI3K protein is involved in the occurrence of hum^m cervical cancer. The ratio of P-AKT and AKT plays a more important role in squamous cancer of the cervix.
出处
《中国医师进修杂志》
2009年第6期1-4,共4页
Chinese Journal of Postgraduates of Medicine
基金
基金项目：浙江省温州市科技计划（Y20060155）
