摘要
目的比较酸性膨润土和有机膨润土的细胞毒性。方法用CCK-8试验、中性红（NRU）试验、乳酸脱氢酶（LDH）试验、凋亡试验和溶血试验来检测2种土的细胞毒性。溶血试验以人的红细胞为靶细胞，暴露剂量为0．0．3125、0．6250、1．2500、2．5000mg／ml，暴露10min。其余4个试验均以人B淋巴细胞系（HMy2．CIR）为靶细胞，暴露剂量为0、10、20、30、60、120、180μg／ml，暴露4h。结果所有剂量2种膨润土的溶血率均明显高于对照组，差异有统计学意义（P〈0．05）。CCK-8试验结果表明，酸性土和有机土剂量分别≥30、20μg／ml时，细胞活性明显低于对照组，差异有统计学意义（P〈0．01）；NRU试验和LDH试验出现类似结果，并均呈剂量—效直关系。180wg／ml酸l生土组与120、180μg／ml有机土组的早期细胞凋亡率明显高于对照组，差异有统计学意义（P〈0．05）。5个体外试验的结果均表明，有机土毒性明显高于酸性土。结论2种膨润土均可诱发细胞凋亡、细胞膜损伤等细胞毒性。有机土细胞毒性明显高于酸性土。
Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite. Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay, neutral red uptake（NRU） assay, lactate dehydrogenase（LDH） leakage assay, apoptosis assay and hemolysis assay. In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0, 0.3125, 0.6250, 1.2500 and 2.5000 mg/ml for ten min. In other four assays, human B lymphoblast cells （HMy2.CIR） served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10, 20, 30, 60, 120 and 180 μg/ml for four h. Results In hemolysis assay, the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control （P〈O.05）; in CCK-8 assay, the cellular activities in acid bentonite group at the doses ≥30μg/ml and in organic bentonite group at the doses 320 μg/ml were significantly lower than that of control （P〈0.01）; the similar results appeared in NRU assay and LDH assay, and the dose-effect relationship was observed in above 4 assays. In apoptosis assay, the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control （P〈0.05）. Moreover, the results of five in vitro assays indicated the cytotoxicity induced by organic bentonite was higher than that induced by acid bentonite. Conclusion Two kinds of bentonite could induce cytotoxicity, such as apoptosis and damage of cell membrane. The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2009年第6期338-340,共3页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
基金项目：浙江省卫生厅青年科技基金（2008QN006）
