摘要
在重组人宋内痢疾杆菌脂多糖抗独特型抗体重链可变区蛋白的表达质粒（ｐＱＥＨｖ）的氨基端插入６个组氨酸，使其对金属螯合层析介质能产生特异性吸附，可用金属螯合亲和层析法进行分离纯化。本研究采用ＮｉＮＴＡ螯合层析介质，以咪唑洗脱表达的蛋白。经ＥＬＩＳＡ检测，纯化的蛋白可特异地与抗宋内痢疾杆菌脂多糖的鼠ｍＡｂ５Ｂ１２发生反应。
Recombinant human Hv(heavy chain variable region of anti idiotypic antibody against antibody of LPS of Shigella sonnei(pQEHv) with a hexahistidine tail attacked to the N terminal of Hv could be easily purified by immobilized metal ion affinity chromatography (IMAC). Immobilized metal ion (Nickel) and elution solution(inidazole) were used in IMAC. The purified protein can react specially with 5B12 monoclonal antibody(anti LPS of Shigella sonnei)in ELISA.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
1998年第2期123-125,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金
关键词
人源抗体
抗独特型
脂多糖
金属螯合层析
抗体
human antibody
anti idiotype
LPS
immobilized metal ion affinity chromatography
