摘要
目的:评估短时受精联合早期补救性卵细胞胞质内单精子注射(ICSI)术在完全受精失败周期的应用结局。方法:10例体外受精-胚胎移植(IVF-ET)周期获取的卵细胞随机分组,分别采用短时受精与过夜受精,比较受精率与胚胎质量。105例常规IVF-ET周期短时受精后,行极体观察,早期判断完全受精失败后行补救性ICSI术,报道临床结局。结果:3h短时受精与过夜受精在受精率与胚胎质量上无明显差异(P>0.05)。短时受精联合早期受精失败补救后,临床妊娠率为53.3%,种植率为38.0%,出生婴儿64名。结论:短时受精联合早期补救性ICSI对常规IVF-ET周期完全受精失败患者的临床结局有明显改善。
Objective ： To evaluate the outcome of the combination of short-period sperm-oocyte coincubation with early rescue intracytoplasmic sperm injection （ICSI） after complete failure of in vitro fertilization （IVF）. Methods： We used short-period sperm-ooeyte coineubation and overnight fertilization for the ooeytes obtained from 10 IVF-ET cycles, and compared the fertilization results and embryo quality. We conducted polar body observation following short-period sperm-ooeyte coincubation for 105 conventional IVF cycles, and evaluated the clinical outcome of early rescue ICSI for total fertilization failure. Results ： No significant differences were noted in fertilization outcome and embryo quality between the 3 h short-period and overnight fertilizations （ P 〉 0.05 ）. The combined strategy achieved a clinical pregnancy rate of 53.3% , an implantation rate of 38.0% , with 64 babies born, of whom 44.8% were taken home. Conclusion： The combination of short-period sperm-oocyte coineubation with early rescue ICSI can significantly improve the clinical outcome in patients who have experienced complete failure of in vitro fertilization.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2009年第6期538-541,共4页
National Journal of Andrology
