摘要
目的在Kamada"二袖套法"基础上采用供肝冷保存时经门静脉再灌注的方法建立稳定的大鼠原位肝移植动物模型,为以后进行肝移植免疫排斥、器官保存、缺血再灌注等进一步的实验研究提供安全、有效的模型制作方法。方法将30只接受供肝移植的SD大鼠随机分为5组(n=6):A为假手术组,开腹后不做任何处理即关腹;B组供肝修整后冷保存期不进行再灌注;C组供肝修整后以5ml生理盐水经门静脉灌注;D组供肝修整后以10ml生理盐水经门静脉灌注;E组供肝修整后以15ml生理盐水经门静脉灌注。然后以改良Kamada"二袖套法"完成大鼠原位肝移植模型,观察各组受体大鼠一般情况、生存情况、肝功能情况和组织病理学改变。结果B组大鼠术后苏醒时间明显长于A组和D组(P<0.05),其存活3d体质量下降程度均高于各组,和D组相比差异明显(P<0.05),B组存活一周体质量增加程度明显小于A、D、E组(P<0.05);B组中位生存时间明显短于A组、C组、D组和E组(P<0.05),D组和E组中位生存时间无明显差异(P>0.05);术后第1、4、7天实验组大鼠ALT显著高于对照组(P<0.05),而D组大鼠ALT值明显低于B组和C组(P<0.05);实验组术后ALB均显著低于对照组(P<0.05),但D组下降程度较低,其ALB仍明显高于B、C、E组(P<0.05),且于术后4d开始升高,E组术后ALB始终处于较低水平。结论通过改进技术改善肝功能,提高了生存率,尤其在冷保存时经门静脉再灌注,在供肝的质量上明显优于不进行灌注组,并应严格掌握灌注液体量,10ml灌注量明显优于其他液体量,能有效地驱除供肝内残留的血液和气泡。
Objective To establish the model of orthotopic liver transplantation in rats by reperfusion via the portal vein during cold preservation on the basis of Kamada＇s ＂two-cuff＂ technique with some modification during operation. Thus, a safe and effective methods were provided for further study, such as immunologic rejection, organ preservation, ischemic and reperfusion injury. Methods Thirty SD recipient rats were divided into 5 groups randomly. The group A only experienced a simulate operation. In the group B, the liver was just preserved after trimming. Five ml of physiological saline was reperfused into allograft through portal vein in the group C. Ten ml of physiological saline was perfused in group D, 15 ml of physiological saline in the E group. And then the general condition, the survival time, the liver function and histopathology examinations were observed. Results The analepsia period in B group was significantly longer than that in group A and D （P〈0.05）. In group B, the decreasing in weight when survived for 3 days was more than group D significantly （P〈0.05）, the middle survival time in A, C, D and E group was obviously longer than that in group B （P〈 0.05）. There was no difference between D and E group （P〉0.05）. The ALT in experimental group was higher than that in the controlled group on 1, 4, 7 days postoperatively （P〈0.05）. The ALB in experimental group was lower than that in the control group on 1, 4, 7 days postoperatively （P〈0.05）, but that in group D had little descend and still higher than that in the rest, was increased on 4 days postoperatively. However, the ALB in group E was always in low level. Conclusion The liver function and survival rate largely improve due to the modified technique. The quality of the allograft, which undewent reperfusion during cold preservation gained an obvious advantage to the routine ones. At the same time, the quantity of the reperfusion liquid should be controlled; 10 ml reperfusion liquid can effectively lustrate the bl
出处
《肝胆胰外科杂志》
CAS
2009年第3期180-183,共4页
Journal of Hepatopancreatobiliary Surgery
基金
浙江省自然科学基金资助项目(X204003)
关键词
肝移植
大鼠
再灌注
动物模型
liver transplantation
rats
repertusion
animal model
