摘要
目的探讨硝基左旋精氨酸甲基酯（Ｌ－ＮＡＭＥ）对小肠上皮细胞辐射防护的机理。方法实验用大鼠ＩＥＣ－６细胞，６０Ｃｏγ射线照射；荧光分光光度法测定培养液上清中一氧化氮（ＮＯ）含量；ＮＡＤＰＨ黄递酶组化法（ＮＤ组化法）和免疫细胞化学法观察一氧化氮合酶（ＮＯＳ）活性变化；ＭＴＴ法检测细胞的生存力。结果ＩＥＣ－６细胞经射线照射后，其培养液上清中ＮＯ含量明显增加，细胞内结构型一氧化氮合酶（ｃＮＯＳ）活性升高，诱导型一氧化氮合酶（ｉＮＯＳ）活性降低，Ｌ－ＮＡＭＥ能明显提高细胞的生存力，ＮＯ底物－左旋精氨酸（Ｌ－Ａｒｇ）部分逆转其作用。结论ＮＯ参与了ＩＥＣ－６细胞的辐射损伤，Ｌ－ＮＡＭＥ对ＩＥＣ－６细胞具有辐射防护作用。
Objective\ To study the effect of L NAME on radiation induced injury of small intestinal epithelial cells 6(IEC 6).Methods \ Rat IEC 6 cells were irradiated with 60~Coγ rays.The content of nitric oxide(NO) in media supernatant of IEC 6 cells was measured by fluorometric method.Nitric oxide synthase (NOS) activity was observed by histochemistry of NADPH diaphase and immunocytochemistry.Cell viability was evaluated by method of MTT.Results \ NO generation by IEC 6 cells was significantly increased after irradiation.The assay of cNOS activity showed that it was stimulated.iNOS activity was inhibited.L NAME was able to increase cell viability which had been reduced by irradiation,but it was reversed partly by pretreatment with NO substrate L Arg.Conclusion\ NO was involved in IEC 6 cell injury induced by radiation and L NAME could provide protective effect on it.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1998年第3期156-158,共3页
Chinese Journal of Radiological Medicine and Protection
关键词
硝基
左旋精氨酸
甲基酯
小肠
上皮细胞
辐射
L NAME\ Small intestines\ \ Epithelial cells\ \ Nitric oxide\ \ Radiation
