摘要
通过PCR方法从Sulfolobus solfataticus P2中扩增到2.6kb的α-淀粉酶基因(SSO1172),将其分别克隆到表达载体pET32a(+)和pPICZaA,并在E.coliRosetta和Pichia pas-toris GS115中进行表达。结果表明α-淀粉酶基因在Rosetta中得到了高效表达,酶活为143.466U/mL;而在GS115中表达量稍低,发酵液酶活力为98.102U/mL。
A thermophilic and acidophilic extracellular α-amylase gene （S SO1172）, amplified by PCR from the genome of Sulfolobus solfataticus P2, was cloned into plasmid pET32a（ ＋ ）, pPICZaA and expressed in Escherichia coli Rosetta and Pichia pastoris GSl15 respectively. The results showed that a-amylase gene was highly expressed in Escherichia coli Rosettawith specific activity 143. 466 U/mL. The α-amylase could be expressed in Pichia pastoris GSl15 and secreted out with the activity of 98. 102 U/mL.
出处
《工业微生物》
CAS
CSCD
2009年第3期56-61,共6页
Industrial Microbiology
基金
科技部农业微生物菌种资源整理整合及共享试点(2005DKA21201-11)。
关键词
硫矿硫化叶菌
嗜热嗜酸α-淀粉酶
融合表达
Sulfolobus solfataticus
thermophilic and acidophilic α-amylase
clone and expression
