摘要
采用高效液相色谱法,以β-环糊精为手性固定相拆分奥丁尼酮洛芬缓释胶囊中酮洛芬对映体,并对流动相中甲醇的含量、流动相的pH值、缓冲盐的浓度等色谱条件对拆分结果的影响进行了探讨.结果表明,在柱温为25℃,pH值为4.0,采用0.01 mol/L的磷酸二氢钾缓冲溶液,甲醇40%(V/V)作流动相时,奥丁尼酮洛芬缓释胶囊中的酮洛芬对映体能达到较好分离.
The method HPLC was developed for the separation of ketoprofen sustained release capsules by β-cyclodextrin chiral stationary phase. The effects of the concentration of methanol for mobile phase,pH and the concentration of salt on chiral separation were studied. The results show that the column temperature 25 ℃ ,0. 01 mol/L potassium dihydrogen phosphate pH = 4.0, containing 40% （V/V） methanol as eluant, ketoprofen sustained release capsules enantiomers were separated.
出处
《鞍山师范学院学报》
2009年第2期24-27,共4页
Journal of Anshan Normal University
基金
辽宁省教育厅科学技术基金资助课题(2024201050)
关键词
奥丁尼酮洛芬
手性固定相
分离
Ketoprofen sustained release capsules
Chiral stationary phase
Separation
