摘要
为了更深入的研究呼吸道合胞病毒F蛋白优势表位的抗原性，采用生物信息学技术，预测呼吸道合胞病毒F蛋白的抗原表位，选取F205-223与F255-278两个小片段表位作为候选研究对象，构建了编码F蛋白两个表住与Balb／c小鼠白蛋白结构域I的融合基因，并在大肠埃希菌BL21（DE3）中融合表达，表达蛋白采用金属亲和层析法纯化，Western blot检测。结果显示，通过大肠埃希菌原核表达的融合蛋白以包涵体的形式表达，采用尿素变性，亲和层析法纯化后可以获得高纯度的融合蛋白。
In order to study the antigenicity of respiratory syncytial virus F glycoprotein epitopes, the F glycoprotein was analyzed using bioinformatics techniques. The two fragment epitopes of F205-223 and F255- 278 were selected for research. The fusion gene encoding F glycoprotein epitopes and Balb/c mouse serum albumin domain Ⅰ was constructed and expressed in E. coli BL21（DE3）. The fusion protein was purified by Ni^2＋ affinity chromatography and detected by Western blot. The results indicated that the expressed protein existed in the form of inclusion body. Using urea denaturation,high purity protein was obtained by affinity chromatography purification.
出处
《动物医学进展》
CSCD
北大核心
2009年第6期36-38,共3页
Progress In Veterinary Medicine
