摘要
【目的】为了明确瓜果腐霉在液体培养基中所产生粗毒素的具有除草活性成分的组成及结构,本研究对具有除草活性毒素成分进行了分离纯化和结构鉴定。【方法】瓜果腐霉培养滤液分别用乙酸乙酯、石油醚、三氯甲烷等不同极性溶剂萃取制备粗毒素,用薄层层析法对粗毒素进行分离,再用高效液相色谱分离制备得到除草活性成分,通过核磁共振谱和红外光谱对其进行结构解析。【结果】用乙酸乙酯萃取得到的粗毒素活性最高;以Rf值为0.19的物质除草活性最强,对马唐的生长抑制作用达到5级;通过化学信息分析表明该成分为邻苯二甲酸二甲酯。【结论】邻苯二甲酸二甲酯是瓜果腐霉在液体培养过程中所产生的组分之一,具有除草活性。
[ Objective ] In order to understand the compsition and structure of herbicidal component from Pythium aphanidermatum, the isolation and structural indentification was researched. [Method] The culture filtrate was extracted by ethyl acetate, petroleum, chloroform with the same volume respectively and the activity of the crude toxin was bioassayed, the toxin was separated by using the method of TLC, then the main fraction was separated by HPLC, and the structure was analyzed by the sepctrum of IR,13C-NMR and ^1HNMR. [Result] The results showed that the ethyl acetate extracts had the strongest herbicidal activity. Using the method of TLC, the bioassay results showed that the extracts with Rf 0.19 had strongest effect on weeds and the inhibition to Digitaria sanguinalis L. and Amaranthus retroflexus L. reached five degree, the component was got using analysis, also was proved to be dimethyl o-phthalate from the sepctrum of IR, 13C-NMR and ^1HNMR. [ Conclusion ] Dimethyl o-phthalate was one of the components from the toxin, and it had herbicidal activity.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第6期1994-2001,共8页
Scientia Agricultura Sinica
基金
河北省自然基金(C2007000464)
国家高技术研究发展计划“863”项目(2006AA10A214)
关键词
瓜果腐霉
毒素
除草活性
结构鉴定
Pythium aphaniderrnatum
toxin
herbicidal
structure identification
