摘要
目的建立测定小鼠血浆神经生长因子(NGF)浓度的同位素示踪沉淀法。方法采用放射性同位素(^(125)I)标记示踪法结合三氯醋酸(TCA)沉淀法测定NGF的浓度。结果本方法的最低检测浓度为2.0 ng/ml,日内、日间变异系数(CV)均小于10%,血浆中NGF在12.5～400 ng/ml浓度范围内均呈良好的相关性,相关系数r=0.9990,血浆中NGF的平均回收率为95.21%。结论同位素标记示踪法与TCA法结合测定血浆NGF,方法稳定、可靠,灵敏度较高,适于NGF血药浓度的测定。
Objective To establish the radioisotope (^(125)I) tracer method combined with trichloroacetic acid (TCA) precipitation for the determination of nerve growth factor (NGF) in mouse plasma. Methods Radioisotope tracer labelling method combined with TCA precipitation was used to determine NGF in mouse plasma. Results The detection limit of the method for NGF in plasma was 2.0 ng/ml. The intra-assay cv (CV) and the inter-assay CV were all less than 10%. The assay linearity was determined over the range of 12.5～400 ng/ ml in plasma and the correlation coefficient was 0.9990. The average recovery of the method for NGF was 95.21%. Conclusions The radioisotope tracer method combined with TCA precipitation is stable, sensitive and reliable. It is suitable for the determination of NGF concentration in mouse plasma.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1998年第3期203-206,共4页
Acta Academiae Medicinae Sinicae
关键词
神经生长因子
同位素
示踪标记法
TCA沉淀法
nerve growth factor
isotope tracer labelling method
TCA precipitation method
