摘要
目的:探讨糖尿病骨骼肌凋亡相关蛋白Bcl-2和Bax表达的变化及意义。方法:选用Wistar大鼠24只,随机分成正常对照组、单纯缺血组、糖尿病组和糖尿病缺血组,每组6只。以四氧嘧啶50mg/kg尾静脉注射制造糖尿病模型。造模后2周结扎右股动脉制造缺血模型。10周后,以免疫组化方法观察腓肠肌中凋亡相关蛋白Bcl-2及Bax表达情况。结果:糖尿病组骨骼肌表现为普遍性萎缩,肌纤维变性坏死。与对照组比较糖尿病可使骨骼肌凋亡相关蛋白Bcl-2表达降低(P<0.05),Bax表达增高(P<0.05)。缺血可使骨骼肌Bax蛋白表达增高(P<0.05),但对Bcl-2表达的影响与对照组比较差异无统计学意义(P>0.05)。糖尿病与缺血对骨骼肌Bcl-2和Bax蛋白表达的影响无交互作用(P>0.05)。结论:糖尿病骨骼肌病变可能与Bcl-2与Bax蛋白比例下降促进细胞凋亡有关。
Objective： To study the relationship of diabetic myopathy with apoptosis related proteins. Methods： Twentyfour Wistar rats were averagely divided into four groups, control group, diabetic group, non-diabetic ischemia group and diabetic ischemia group. The diabetes rat model in experimental group was produced by injection of alloxan 50 mg/kg via the tail vein. After 10 weeks of experimental term, the expressions of Bcl-2 and Bax in gastrocnemius were studied by immunohistochemistry. Results：The morphometric changes of skeletal muscle in experimental diabetic rats were characterized primarily by widespread myatropby, muscle fiber denaturalization and necrosis. The diabetic condition decreased the expression of Bcl-2 （P 〈 0.05） and increased the expression of Bax （P 〈 0.05）. The is chemia increased the expression of Bax （P 〈 0.05）, but its effect on the expression of Bcl-2 was not statistically significant （P 〉 0.05）. The ischemia and diabetic condition has no coordinated interaction on the expressions of Bcl-2 and Bax in gastrocnemius （P 〉 0.05）. Conclusion： The decreased rate of Bcl-2 and Bax contributed to the apoptosis of skeletal muscle cells, which might aggravate muscular lesion.
出处
《天津医药》
CAS
北大核心
2009年第6期492-494,I0001,共4页
Tianjin Medical Journal
