摘要
[目的]研究兔消化系统中内源性纤维素酶及其酶学性质。[方法]取兔子消化系统中的胃、十二指肠、小肠和大肠,用DNS法测定纤维素酶活并对其性质进行研究。[结果]结果表明,胃、十二指肠、小肠和大肠纤维素酶活性最高的底物浓度均是0.625%,最适显色时间5 min,最适pH值分别是4、6、7、6,最适温度是48℃。[结论]该研究结果为兔饲料及其应用研究提供一定的理论基础。
[ Objective ] The purpose of this research was to study the endogenous cellulose and its enzymatic character in digestive system of rabbit. [ Method ] The stomach, the duodenum, the small intestine and the large intestine in digestive system of rabbit were taken to study its zymology activeness. DNS menthod was used to analyze the effects of determination conditions on results of measurements. [ Result ] The resuits indicated that under the different conditions,bodies cellulase activities were different. The stomach, the duodenum, the small intestine, the large intestine most suitable substrate concentration were 0.625%, the most suitable colored time was 5 min, most suitable pH value was 4, 6, 7 and 6, respectively. The stomach, the duodenum intestines, the small intestine and the large intestine optimum temperature was 48 ℃. [ Conclusion ] This research result will provide the theoretical references for rabbit foodstuff and application of additive.
出处
《安徽农业科学》
CAS
北大核心
2009年第16期7456-7457,7528,共3页
Journal of Anhui Agricultural Sciences
基金
黑龙江省教育厅科学技术研究项目(11541387
11511447
11531425)
黑龙江省自然科学基金资助项目(C200624)
关键词
纤维素酶
酶活
酶学性质
兔
Cellulase
Cellulase activity
Enzymatic character
Rabbit
