摘要
[目的]研究从转人白细胞介素-12(hIL-12)基因马铃薯中纯化的hIL-12的生物学活性。[方法]MTT法检测纯化的hIL-12体外抗肿瘤作用。[结果]纯化的hIL-12在体外对NK敏感肿瘤细胞K562有杀伤作用,而野生对照组无作用。[结论]试验中从转hIL-12基因马铃薯中纯化的hIL-12具有生物学活性。
[ Objective] The aim was to investigate the bioactivity of human interleukin 12 （hIL-12） purified from transgenic potatoes. [ Meth- ods] The anti-tumor function of purified hIL-12 was detected by MTY method. [ Result] The purified hIL-12 was able to enhance killing effect on K562, while wild potatoes were inactivity. [ Conclusion] hIL-12 purified from transgenic potatoes is bioactive in the test.
出处
《安徽农业科学》
CAS
北大核心
2009年第16期7334-7334,7347,共2页
Journal of Anhui Agricultural Sciences
基金
贵州省科委重大攻关项目(2004NGZ002)
贵州省教育厅重点项目(2004118)
