摘要
目的探讨人良性脑膜瘤细胞的热敏感性及其机制。方法15例人脑膜瘤原代培养细胞进行温水加热体外实验。采用Western blot法检测HSF1蛋白表达;免疫组织化学法检测HSF1、HSP27、HSP70和HSP90蛋白表达;MTT法检测细胞增殖抑制率、TUNNEL法检测细胞凋亡率。结果15例人良性脑膜瘤在44℃温水加热60min后细胞增殖抑制率仅为(8.33±0.18)%,凋亡率(%)为(2.4±0.16)%。温水加热后,人脑膜瘤增殖能力没有受到抑制,没有诱导细胞凋亡或细胞坏死(P>0.05)。在热刺激下,HSF1蛋白发生活化,由单体(80kD)转化为三聚体(260kD)。加热后6h出现HSP27、HSP70与HSP90表达明显增加,持续24h高表达,显著高于加热前对照组(P<0.05)。结论人脑膜瘤热敏感性低,单纯温水加热(42～44℃)无抑制细胞增殖和增加细胞凋亡的作用。其机制可能是在加热刺激下,人脑膜瘤细胞HSF1蛋白发生三聚体活化,诱导型HSP27、HSP70和HSP90在加热后24h内持续高表达,保护和修复了热损伤的人脑膜瘤细胞。
[Objectives] To observe the heat sensitivity of human miningioma and to explore the underlying mechanisms. [Methods] The in vitro experiments of warm heat hyperthermia were conducted in 15 cases of human meningioma primary culture cell, so as to detect proliferation inhibit rate, apoptosis rate and the change of HSF1, HSP27, HSP70 and HSP90 protein by IHC, western blot, MTT method, TUNNEL method. [Results] 15 cases of human meningioma short term primary culture cell was not sensitive to warm water hyperthermia, the cell prolifera- tion inhibit rate was only （8.33±0.18）% and apoptosis rate was low extremely under the condition of heat test （44 ℃, 1 hour）, which was not obvious difference with control group. After warm water hyperthermia, HSF1 protein did not increase but activated and converted from monomeric form to trimerization form. After hyperthermia, the expression of HSP27, HSP70 and HSP90 protein increased very much and kept on high levels from 6 hours to 24 hours, which was significant difference with control group. [Conclusions] The heat sensitivity of human meningiomas was low, warm water hyperthermia did not suppress cell proliferation and did not increased cell apoptosis. The mechanism maybe persistently high expression levels of induced HSP27, HSP70 and HSP90 by trimerization of HSF1 that proretted and repaired human meningiomas.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第10期1506-1510,共5页
China Journal of Modern Medicine
基金
福建医科大学青年教师科研基金项目(No:FJGXQ04022)
