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联合检测抗核小体、抗组蛋白和抗双链DNA抗体在SLE中的意义

The significance of combinative detecting of anti-nucleosome, anti-histone and anti-double stranded DNA in systemic Lupus erythematosus
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摘要 目的探讨抗核小体抗体、抗组蛋白抗体和抗双链DNA抗体联合检测在SLE中的意义。方法采用免疫斑点法对199例SLE患者,309例其他弥漫性结缔组织病(CTD)患者和72例正常组进行上述3个抗体的联合检测,并与SLE患者临床及实验室指标进行相关性分析。结果核小体抗体在SLE组、CTD组和正常组中的阳性率为47.24%、0.97%和0.00%;抗组蛋白抗体为41.71%、0.97%和0.00%;抗双链DNA抗体为33.17%、0.65%和0.00%,上述各组内阳性率差异均有统计学意义(P〈0.05)。抗核小体抗体、抗组蛋白抗体和抗双链DNA抗体在SLE组中的特异性分别为96.91%、96.51%和97.06%;任何两种或三种抗体同时阳性对SLE的特异性为100%。三种抗体在SLE活动组中的阳性率均明显高于非活动组(P〈0.01);抗核小体抗体与WBC、HB、C3、C4、胸膜炎等指标间具有相关性(P〈0.05)。结论联合检测抗核小体抗体、抗组蛋白抗体和抗双链DNA抗体对SLE中有较好的敏感性和特异性,抗核小体抗体的敏感性最高且与SLE病情活动密切相关。 Objective To evaluate the significance of anti-nucleosome antibody (AnuA), anti-Histone antibody(AHA) and anti-double stranded DNA(ds-DNA) in systemic lupus erythematosus (SLE). Methods In 199 patients with SLE, 309 patients of other connective tissue disease(CTD) and 72 healthy people, serum AnuA,AHA and anti-ds-DNA were tested by immunodotting assay. Results The positive rates of AnuA, AHA and anti-ds-DNA in patients with SLE,CTD and healthy people were 47.24% ,0. 97% and 0. 00% ; 41.71%, 0. 97% and 0. 00% ; 33.17% ,0. 65% and 0. 00%. The difference of positive rates in three groups had signifi- cant statistic value ( P 〈 0. 05 ). The specificity of AnuA, AHA and anti-ds-DNA in patients with SLE were 96. 91% ,96. 51% and 97.06%. Any two antibodies or all the three antibodies were positive in patients with SLE simultaneous, the specificity was 100%. The positive rates of AnuA, AHA and anti-ds-DNA in patients with SLE who were in active group were obviously higher than that in non-active group. There were significant correlations between AnuA and some factors such as WBC, HB, C3 , C4, pleurisy in SLE. Conclusion AnuA, AHA and anti- ds-DNA has high specificity and good sensitivity in SLE. AnuA has the highest sensitivity and might be a marker of the disease activity for the patients with SLE.
出处 《中国临床实用医学》 2009年第6期13-14,共2页 China Clinical Practical Medicine
关键词 抗核小体扰体 抗组蛋白抗体 抗DSDNA抗体 系统性红斑狼疮 Anti-nucleosome antibody Anti-Histone antibody Anti-ds-DNA Systemic lupus erythema-tosus
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