摘要
本研究构建了含人ＰＤＧＦＢ链基因不同上游序列的荧光素酶报告基因ｐＳＩＳ－１７５８／＋４３Ｌｕｃ和ｐＳＩＳ－４０２／＋４３Ｌｕｃ，经与ｐＳＶβＧａｌａｃｔｏｓｉｄａｓｅＣｏｎｔｒｏｌＶｅｃｔｏｒ共转染血管内皮细胞后，测定了基础水平和ＴＮＦα作用下细胞裂解液中的荧光素酶和β半乳糖苷酶活性。结果表明：在ＴＮＦα作用下，ＰＤＧＦＢ链基因上游序列上调内皮细胞荧光素酶的表达，其主要调控区可能位于－１７５８／－４０３ｂｐ之间。
Recombinant luciferase repoter genes(pSIS 1758/+43 Luc and pSIS 402/+43 Luc)containing different upstream sequences( 1758/+43bp and 402+43bp,respectively)of human PDGF B chain gene have been constructed.After these reporter genes and pSV β galatosidase vector were cotransfected into human vascular endothelial cells,the activities of the cellular luciferase and β galatosidase were measured under either normal state or TNFα treatment.The results suggest that under TNF a treatment,presence of upstream sequences of PDGF B chain gene up regulates luciferase expression in endothelial cells and a TNFα induced positive regulatory element may be restricted to 1758/ 403bp.
出处
《基础医学与临床》
CSCD
1998年第3期68-72,共5页
Basic and Clinical Medicine
基金
国家自然科学基金
