摘要
目的:探讨银杏叶提取物(ginkgo biloba extract,GBE)对肝肺综合征(hepatopulmonary syndrome,HPS)大鼠血管扩张因子内皮素-1(endothelin-1,ET-1)、一氧化氮(nitric oxide,NO)、降钙素基因相关肽(calcitoningene-related peptide,CGRP)的影响。方法:雄性成熟SD(Spraque Dawley,SD)大鼠32只,随机平均分为4组:模型组、正常对照组、GBE低剂量组、GBE高剂量组,采用胆总管结扎术(commor bile duct ligation,CBDL)成功制备大鼠HPS模型,喂养3周后统一处死,放射免疫分析法检测大鼠血浆和肝组织、肺组织匀浆中ET-1和CGRP的水平,利用硝酸还原酶法检测大鼠血清和肝、肺组织匀浆中NO的水平。结果:在大鼠HPS形成过程中,模型组、GBE低剂量组、GBE高剂量组血浆(血清)和肝、肿组织匀浆中ET-1、NO、CGRP水平显著升高,经GBE治疗后显著下降,且GBE高剂量组更为显著。结论:GBE可降低HPS大鼠血浆(血清)和肝、肺组织匀浆中ET-1、NO和CGRP水平,且高剂量比低剂量的治疗效果明显。
Objective：To explore the effects of ginkgo biloba extract on endothelin -1 ,nitric oxide and ealeitonin gene - related peptide in HPS rats. Methods ： Thirty two male SD rats were randomly and averagely divided into 4 groups ： model group ; nomal control group; GBE low-dosage group ; GBE high-dosage group. HPS model was induced by CBDL. They were all sacrlificed alter 3 weeks. The concentrations of ET - 1 and CGRP in plasma,liver and lung tissiles were detected by radioimmunoas-say（RIA） and the NO content in serum ,liver and lung tissues was measured with nitrate reductase method. Results：During the pathogenesis of HPS,the levels of ET - 1, No and CGRP in plasma or serum,liver and lung tissues in model group, GBE low-dosege group and GBE high-dosage group were increased and strikingly reduced after treated by GBE, especially in GBE high-dosage group. Conclusion ： GBE can reduced the levels of ET - 1, NO and CGRP in plasma or serum,liver and lung tissues in HPS rats,and it＇s effect of treatment in GBE high-dosage was more obvious than that in GBE low-dosage.
出处
《中华中医药学刊》
CAS
2009年第6期1305-1307,共3页
Chinese Archives of Traditional Chinese Medicine
