摘要
为研制组织工程小口径血管,以良好生物相容性和生物可降解性的聚乳酸(PLA)、丝素蛋白、明胶为原料,通过静电纺丝法,以高速旋转的滚轴为收集装置,构建了外层为PLA纤维和内层为丝素明胶纤维的PLA/丝素明胶复合管状支架(直径为4.5 mm)。采用扫描电镜观察该管状支架的形貌结构;测定其孔隙率及生物力学性能,并在该支架上进行人脐静脉内皮细胞(HUVECs)培养实验。结果表明:PLA/丝素明胶复合管状支架具有较高的断裂强度和较好的柔软性,爆破强度远高于人体的正常血压;支架具有多孔结构,SEM照片显示HUVECs在支架上分化、增殖、生长状态良好。
To develop a small-diameter tissue engineering blood vessel, the biocompatible and biodegradable PLA, silk fibroln and gelatin were used as materials, polylactide/silk fibroin-gelatiu composite tubular scaffold with 4.5 mm diameter was fabricated via electrospinning, which was performed on a high speed rotating mandrel. The composite tubular scaffold was composed of a PLA fibrous outside layer and a silk fibroin-gelatin fibrous inside layer. The morphology of the scaffold was observed by SEM; porosity and biomechanical properties of the scaffold were characterized, HUVECs were cultured on the scaffold. The results indicate that PLA/silk fibroin-gelatin composite tubular scaffold shows a high breaking tenacity and preferable pliability, the burst strength of the scaffold is higher than normal blood pressure in human body; the scaffold has a lacunaris structure, SEM image shows HUVECs could grow and proliferate on the scaffold.
出处
《纺织学报》
EI
CAS
CSCD
北大核心
2009年第6期6-9,14,共5页
Journal of Textile Research
基金
江苏省高校重点实验室开放研究课题(KJS0817)
关键词
静电纺丝
管状支架
组织工程血管
PLA
丝素蛋白
明胶
electrospinning
tubular scaffold
tissue engineering blood vessel
PLA
silk fibroin
gelatin
