摘要
初产供体母羊(14月龄)德国美利奴15只、波德代15只、无角陶赛特18只分别用孕酮(CIDR)+促性腺激素(PMSG)+促卵泡素(FSH)进行处理,受体小尾寒羊母羊(2胎)124只用CIDR+PMSG进行处理,处理期测定供、受体外周血液中生殖激素孕酮(p4)、雌二醇(E2)、促卵泡素(FSH)和促黄体素(LH)的动态变化。结果显示:3个供体品种和受体各自发情同期化水平和效果达到非常理想的状态,但供、受体发情同步差相距1 d。黄体数德国美利奴(7.71)显著高于陶赛特(5.19)(P<0.05);冲卵数德国美利奴(5.50)显著高于陶赛特(3.44)(P<0.05);可用胚胎率德国美利奴、波德代、陶赛特分别为70.13%、55.74%、78.18%,陶赛特显著高于波德代(P<0.05);德国美利奴、波德代、陶赛特胚胎移植受胎率分别为55.26%、54.17%、42.31%,德国美利奴显著高于陶赛特(P<0.05)。处理期供体和受体生殖激素动态变化的幅度大,第13天供、受体血清中p4达到峰值,小尾寒羊分别高于德国美利奴(5.37 ng/mL)、波德代(6.14 ng/mL)和陶赛特(5.37 ng/mL);供体血清中E2于第14天开始发情达到峰值,小尾寒羊于第15天开始发情达到峰值;供体血清中FSH于第13天达到峰值,小尾寒羊第14天达到峰值;供体血清中LH于第14天开始发情达到峰值,小尾寒羊血清中LH于第15天开始发情达到峰值。
Fifteen German Mutton Merino （14 male） and eighteen Poll Dorset （14 months, PMSG＋ FSH treatment, 124 Small-Tailed months, female）, fifteen Borderdale （14 months, fefemale） were selected as donor and with CIDR ＋Han sheep were selected as the recipient and with ＂CIDR ＋ PMSG＂ treatment to study the changes of the progesterone （P4）, estradiol （E2）, Follicle- Stimulating-Hormone （FSH） and luteinizing-hormone （LH）. The results showed that the donor sheep and the recipient sheep reached a very ideal estrus synchronization state respectively. The luteal number of German Mutton Merino （7.71） was significantly higher than that of Dorset （5.19, P（ 0.05）. The number of eggs （including zygote and non-zygote） got from German Mutton Merino （5.50） was significantly higher than that of Dorset （3.44, P〈0.05） the rate of embryos effective of German Mutton Merino, Borderdal and Dorset were 70.13%, 55.74% and 78.18% respectively, and Dorset was significantly higher than that of Borderdale （P〈0.05） ; German Mutton Merino, Borderdale, Dorset embryo transfer pregnancy rate was 55.26%, 54.17% and 42.31% respectively, German Mutton Merino was significantly higher than that of Dorset （P〈0.05）. During treatment of donor and recipient procreation hormone dynamic variation, for on the 13th day, the recipient reached its serum p4 peak, the Small-Tailed Han sheep were higher than those of German Mutton Merino, Borderdale and Dorset. The E2 in serum of Donor reached its peak on the 14th day. The Small-Tailed Han sheep reached its peak on the 15th day, The FSH in serum of donor on the 13th day reached its peak and Small-Tailed Han sheep reached the peak on the 14th day. The LH in serum of donor reached its peak on the 14 day and Small-tailed Han sheep serum LH reached its peak on the 15th day.
出处
《草业科学》
CAS
CSCD
北大核心
2009年第6期126-131,共6页
Pratacultural Science
基金
甘肃省农业生物技术研究与应用开发项目(GN-SW-2005-11)
关键词
生殖内分泌调控
胚胎移植
绵羊
procreation endocrine regulation
embryo transfer
sheep
