摘要
以人心果幼嫩叶片组织为材料,研究了酶液组合、酶解时间、酶液中甘露醇含量、pH值、温度等因素对其原生质体分离的影响,结果表明:以3%纤维素酶R-10+0.4%果胶酶Y-23为混合酶液,0.6 mol.L-1甘露醇为渗透压稳定剂,pH 5.6的酶液组合,在28℃的黑暗条件下振荡,酶解时间2 h,分离出的原生质体产量最佳,可达到12.4×106个.g-1。
The tender leaves of Manilkara zapota were used as experiment materials and the influences of enzyme solution combinations, zymohydrolysis time and mannitol concentration in the enzyme solution , pH value, and temperature on protoplast isolation were studied. The results showed that the highest yield （12.4 × 10^6/g） was obtained by treating the leaves with a mixture of 3 % of cellulose enzyme R-10 ＋ 0.4 % of pectase Y-23 with pH value of 5.6 for 2h of zymohydrolysis time at the temperature of 28℃, and on the oscillation condition with 0.6 mol. L^-1 of mannitol as the osmotic pressure stabilizer.
出处
《福建林业科技》
2009年第2期127-130,共4页
Journal of Fujian Forestry Science and Technology
关键词
人心果
原生质体分离
原生质体产量
Manilkara zapota
protoplast isolation
protoplast yield
