摘要
本研究通过对几种酶消化后人羊膜基膜(HABM)超微结构改变的观察,发现肝素酶Ⅱ可使HABM表层呈微绒毛状改变,并使基膜致密层和透明层变薄及小空洞等改变。推测肝素酶Ⅱ消化后的HABM,可使表面积相对增加,细胞易于附着。肝素酶Ⅱ的作用,主要是使HABM表面的硫酸乙酰肝素降解,使其内的基板蛋白暴露出神经细胞附着和生长位点,从而能使神经细胞的生长增加150％。而肝素酶Ⅰ、胶原酶Ⅰ和硫酸软骨素酶AC消化后的HABM,失去了支持神经细胞附着和生长的各种因素,结果使神经细胞的生长下降。本文从形态学的角度,支持经肝素酶Ⅱ消化后的HABM是神经细胞粘附和生长的理想支持物。
Using enzymatic digestions on human amniotic basement membrane (HABM), it was observed ultrastructurally that heparinase Ⅱ digestion resulted in formation of microvilli on the surface of HABM and thinning and vesiculation of lamina densa and lamina lucida layers. This suggested that the heparinase Ⅱ-digested HABM had increased its surface area for more efficient cell attachment. The main action of heparinase Ⅱ is to digest heparan sulphate on HABM surface, which leads to exposure of more sites of laminin for cell attachment and neurite outgrowth; therefore heparinase Ⅱ-digested HABM can increase neuronal growth to 150%. For those HABM digested by heparinase Ⅰ, collagenase Ⅰ, and chondroitinase AC, they lost most of the sites for attachment and neurite outgrowth and as a consequence, the growth of neurons decreased. This morphological study supports heparinase Ⅱ digested HABM as an ideal substratum for neuronal attachment and growth.
出处
《解剖学报》
CAS
CSCD
北大核心
1990年第3期286-289,共4页
Acta Anatomica Sinica
基金
Croucher基金
香港大学和医学院研究基金~~
关键词
人羊膜基膜
肝素酶
酶消化
电镜
Human amniotic basement membrane (HABM)
Heparinase
Enzymatic digestion
Electron microscopy
