摘要
目的:探讨一种提取高纯度和一定数量的嗅鞘细胞( OECs)的新方法,并研究其与神经支架修复材料PLGL的生物相容性。方法:从新生的Wister大鼠身上分离制备嗅鞘细胞并与PLGL共同培养。测量其接触角、吸附率、存活率等。结果:PDLLA的接触角( 84.5°±1.5°)明显大于PLGL的接触角(52.6°±0.8°)。PLGL的吸附率(80%)明显高于PDLLA(57%)。PLGL的细胞存活率( 88%)明显高于PDLLA(76%)。结论:PLGL比PDLLA具有更好的亲水性、存活率以及良好的细胞生长状况。
Objective To investigate a new way to yield plenty of high purity olfactory ensheathing cells （OECs） and its biocompatibility with appropriate scaffolds. Methods OECs were prepared from neonatal Wister rats and co-cultured with poly [ LA - co - （ Glc - ah - Lys） ] （PLGL）. Its contact angle, adherent rate, and activity rate were tested. Results The contact angle of poly （ D, L - lactic acid） （PDLLA） （84.5° ± 1.5°） was significantly higher than that of PLGL （52.6° ±0.8°）, the adherent rate of PLGL （80%） was significantly higher than that of the PDLLA （57%）, and the activity rate of PLGL （88%） was much higher than that of the PDLLA （76%）. Conclusion PLGL possesses better hydrophilicity and biocompatibility than PDLLA, and it can provide a better cell growth circumstance which is helpful for the effective treatment of nerve injury.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2009年第5期382-387,共6页
Journal of Central South University ：Medical Science
基金
supported by the Key Project of Chinese National Programs for Fundamental Research and Development(2005CB623905)
关键词
嗅鞘细胞
脊神经损伤
PLGL
神经修复材料
生物相容性
olfactory ensheathing cells （OECs）
spinal cord injury （SCI）
poly [ LA-co-（Glc-alt-Lys） ] （PLGL）
nerve repairing scaffold
biocompatibility
