摘要
伤寒沙门菌和副伤寒沙门菌是引起肠热症的病因,在发展中国家是威胁人们健康的重要问题。两者临床症状难于区分,确切的病原学检测及鉴别诊断非常必要。本文回顾了伤寒从分离培养、血清学方法到PCR方法,以及鉴别伤寒和甲型副伤寒沙门菌感染的几种多重PCR方法。这几种多重PCR方法分别以扩增rfb基因簇中伤寒和甲型副伤寒沙门菌各自特异基因,扩增O抗原、Vi抗原、鞭毛蛋白编码基因及扩增多个特异的蛋白编码序列达到鉴别诊断的目的。
Salmonella Typhi and Salmonella Paratyphi are the pathogens to cause enteric fever,which remains serious threat to human heath in developing countries.It is difficult to distinguish typhoid fever from paratyphoid fever based on clinical symptoms,so the etiological detection and differential diagnosis are needed.This paper summaries laboratory detection methods for the diagnosis of typhoid fever,including pathogen isolation,serological test and PCR,and several multiplex PCR for the differential diagnosis of typhoid fever and Salmonella Paratyphi A infection.Multiplex PCR can be used for the amplification of rfb cluster gene,O antigen,Vi antigen,flagellin coding genes and other specific genes of Salmonella Typhi and Salmonella Paratyphi A for the differential purpose.
出处
《疾病监测》
CAS
2009年第4期293-297,共5页
Disease Surveillance
关键词
伤寒
甲型副伤寒
诊断方法
typhoid fever
Salmonella Typhi and Salmonella Paratyphi A
laboratory diagnosis
