摘要
目的:研究天然有效成分氧化苦参碱(Oxy)和粉防己碱(TET)对鼻咽癌耐药细胞株HNE-1(200)的耐药逆转作用.方法:以鼻咽癌耐药细胞株HNE-1(200)为研究对象,用MTT法测定无毒剂量的Oxy和TET作用后HNE-1(200)耐药细胞对常用抗肿瘤药物顺铂(DDP),5-氟尿嘧啶(5-Fu),长春新碱(vincristine,VCR),阿霉素(Adriamycin,ADM)的增殖抑制率和IC50,并进一步计算其逆转指数.结果:无毒剂量的TET和Oxy分别与4种抗肿瘤药物联合应用后,抗肿瘤药物对耐药细胞的增殖抑制率增加.TET逆转HNE-1(200)细胞对DDP,5-Fu,VCR,ADM的耐药指数分别为2.25,2.1,3.19,2.27;Oxy逆转HNE-1(200)细胞对DDP,5-Fu,VCR,ADM的耐药指数分别为1.59,1.26,2.28,1.26.结论:低毒浓度的天然药物Oxy和TET对鼻咽耐药细胞株HNE-1(200)具有一定的耐药逆转作用.
AIM： To study the reversal effects of natural substances oxymatrine and tetrandrine on multidrug resistance in human nasopharyngeal carcinoma muhidrug resistance HNE-1 （200） cell line. METHODS： Human nasopharyngeal carcinoma multidrug resistance HNE-1 （：200） cell line was used as the subject. MTt reduction assay was used to investigate the drug interaction of oxymatrine, tetrandrine, cisplatin （ DDP）, 5-Fluorouracil （ 5-Fu ）, vincristine（VCR） and adriamycin （ ADM ） on HNE-1 （ 200 ） cell line. IC50 and RI were detected. RESULTS： After non-toxic dose of tetrandrine was interacted with DDP, 5-Fu, VCR and ADM, the cell inhibition rate of the four drugs increased. The resistance index （RI） of tetandrine with DDP, 5-Fu, VCR and ADM was 2.25, 2. 1, 3. 19 and 2.27 respectively. The RI of oxymatrine with DDP, 5-Fu, VCR and ADM was 1.59, 1.26, 2.28 and 1.26 respectively. CONCLUSION： Oxymatrine and tetrandrine can reverse the muhidrug resistance of HNE-1 （200） cell line to DDP, 5-Fu, VCR and ADM.
出处
《第四军医大学学报》
北大核心
2009年第11期967-970,共4页
Journal of the Fourth Military Medical University
基金
重庆市卫生局中医处资助课题(渝中医[2003]32号2003-B-90)
