摘要
目的联用免疫磁珠和实时荧光定量RT-PCR(FQ RT-PCR)检测前列腺癌(PCa)患者外周血PSA mRNA,诊断微转移,评价其临床应用价值。方法抽取53例PCa患者、30例良性前列腺增生(BPH)患者及5例健康对照的外周血,分离单个核细胞,与事先已与鼠抗人前列腺特异膜抗原(PSMA)单抗预孵育的包被人抗鼠IgG抗体的磁珠作用,富集PSMA阳性细胞。实时FQ RT-PCR检测富集细胞的PSA mRNA。结果5例健康对照者均为阴性;30例BPH患者中1例阳性;53例PCa患者中有23例(43.4%)阳性,其中ECT证实的27例骨转移癌患者中,有17例(63%)阳性。结论免疫磁珠法联合实时FQ RT-PCR方法检测PSA mRNA判断PCa患者外周血微转移,有助于转移PCa的早期诊断。
Objective To detect peripheral blood micrometastasis in patients with prostate cancer （PCa） by combination of immunomagnetic separation and fluorescence quantitative reverse transcription polymerase chain reaction （ FQ RT-PCR） and to evaluate its clinical application. Methods Blood samples were obtained from 53 PCa patients and 30 benign prostatic hyperplasia （BPH） patients and 5 healthy blood donors. Isolated peripheral blood mononuclear cells, including cancer cells, were incubated with anti-mouse IgG-coated immunomagnetic beads which had been preincubated with mouse anti-human prostate specific membrane antigen （PSA） monoclonal antibody. Ceils bound to the beads were analyzed for PSA mRNA expression by FQ RT-PCR. Results None of the blood donors was positive for PSA mRNA. Only one of the 30 BPH patients had PSA mRNA expression. Of the 53 PCa patients, 23 （43.4%） were positive for PSA mRNA. Of 27 PCa patients who had bone metastases confirmed by ECT, 17 （63%） had detectable PSA mRNA. Conclusions Combination of immunomagnctic separation with FQ RT-PCR to diagnose peripheral blood micrometastasis is useful to detect micrometastasis PCa in early stage.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2009年第3期177-179,共3页
Chinese Journal of Clinical Laboratory Science
关键词
免疫磁珠分选
前列腺癌
微转移
前列腺特异抗原
逆转录-聚合酶链反应
immunomagnetic separation
prostatic neoplasm
micrometastasis
prostate-specific antigen
reverse transcriptase polymerase chain reaction
