摘要
目的探索雌性骨髓干细胞(FBMSCs)离体培养条件下是否表达生殖干细胞减数分裂启动标志基因Stra8,判断其是否有向卵母细胞样细胞分化的能力。方法全骨髓贴壁法分离青年雌性SD大鼠FBMSCs,传代培养第3代FBMSCs。将第3代FBMSCs随机分为2组:(1)对照组应用DMEM+15%胎牛血清培养8d;(2)全反式维甲酸(ATRA)组在对照组基础上加入终浓度为10-5mol/L的ATRA。用RT-PCR方法检测2组Oct4、Vasa、Stra8 mRNA的表达。结果对照组和ATRA组连续培养8d都可检测到Oct4、Vasa、Stra8 mRNA在诱导分化的FBMSCs中的表达。结论FBMSCs中具有能向卵母细胞样细胞分化的多能干细胞;减数分裂启动标志基因Stra8不仅在雌性胚胎生殖干细胞表达,也在成年FBMSCs离体分化中表达。
Objective The aim is to investigate whether adult female bone marrow stem cells （FBMSCs）cultured in vitro condition are able to express stra8 （a specific expression gene in mammalian embryonic ovarian germ cell＇s transition from mitosis to meiosis） and deduce that whether the FBMSCs have the potentia to differentiate into oocyte. Methods FBMSCs were separated from adult female SD rat bone marrow. FBMSCs from the 3nd passage divided two groups in random： （1）Control group： FBMSCs were cultured in DMEM supplemented with 15 % fetal bovine serum for 8 days. （2）all-trans retinoic acid（ATRA）group..FBMSCs were cultured additional with 10^-5 mol/L ATRA in control group culture medium for 8 days. RT-PCR was performed to detect the expression of germ stem cell marker gene Oct4, Vasa and Stra8 mRNA of the 2 groups. Results The FBMSCs of both ATRA group and control group expressed germ stem cell marker gene Oct4 and Vasa and Stra8 mRNA by RT-PCR from 1 to 8 days after induction differentiation. Conclusions These results suggest that adult FBMSCs contain pluripotent stem cells that could differentiate into oocyte. Stra8 （a specific expression gene in mammalian embryonic ovarian germ cell＇s transition from mitosis to meiosis）is not only expressed in embryonic germ cells of ovary, but also can be expressed in adult FBMSCs cultured in vitro condition.
出处
《江西医学院学报》
CAS
2009年第2期1-5,共5页
Acta Academiae Medicinae Jiangxi
基金
国家自然科学基金(30360032)
江西省教育厅科研项目(GJJ09103)
