摘要
目的：研究槲皮素、芦丁、维生素Ｃ（ＶＣ）、维生素Ｅ（ＶＥ）对超氧阴离子自由基的清除作用。方法：利用邻苯三酚自氧化体系产生超氧阴离子自由基，用单扫示波极谱法进行检测。结果：槲皮素与芦丁都能有效地清除超氧阴离子自由基（Ｏ２），但芦丁的清除作用比槲皮素的弱些，它们的ＩＣ５０值分别为１７．８μｍｏｌ·Ｌ－１和１６．３μｍｏｌ·Ｌ－１。二者清除能力强弱的差别可从其结构特征方面进行合理地解释。相同浓度的ＶＣ和ＶＥ对Ｏ２的抑制率分别为７６．８％和２５．７％。结论：槲皮素和芦丁均为黄酮类化合物，能够有效地清除超氧阴离子自由基，用电化学方法可以直接检测其对自由基的清除作用，在此体系中，ＶＣ清除Ｏ２的作用强于ＶＥ。
OBJECTIVE: To investigate the anti superoxide anion radical effect of quercetin, rutin, VC, VE. METHODS: The single sweep oscillopolarography method was applied to determine the O NFDC3 2 that was produced by the autoxidation of pyrogallol. RESULTS: Quercetin and rutin inhibited the formation of O NFDC3 2 effectively, however, the scavenging ability of rutin was weaker than that of quercetin, and their IC 50 was 17.8 μmol·L -1 and 16.3 μmol·L -1 respectively. The difference of scavenging effect of these two compounds can be interpreted from their chemical structures. The inhibitory rates of VC and VE at the same concentration were 76.8%, 25.7% respectively. CONCLUSIONS: Quercetin and rutin are flavonoid and effective superoxide radical scavengers. The scavenging effect of flavonoids on superoxide anion radical can be determined directly by electrochemistry method. The scavenging effect of VC on O NFDC3 2 is stronger than that of VE in this system.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1998年第6期363-365,共3页
Chinese Pharmaceutical Journal
基金
国家自然科学基金
中国科学院应用化学研究所电分析化学开放实验室基金
关键词
槲皮素
超氧阴离子
自由基
清除
单扫示波极谱法
quercetin, rutin, superoxide anion radical, scavenging effect, single sweep oscillopolarography
