摘要
用分光光度法和放射免疫分析法分别检测ＮＯ及ｃＧＭＰ水平，同时观察ｌ丁基苯酞（ｌＮＢＰ）和ｄ丁基苯酞（ｄＮＢＰ）对原代培养的胎大鼠皮层神经细胞外液ＮＯ及胞浆内ｃＧＭＰ水平的影响。结果表明，ｄＮＢＰ（０１～１００μｍｏｌ·Ｌ－１）对低糖低氧条件下或含Ｎ甲基Ｄ门冬氨酸（ＮＭＤＡ）或含ＫＣｌ的培养基中皮层神经细胞外液ＮＯ及细胞内ｃＧＭＰ水平明显升高，而ｌＮＢＰ（０１～１００μｍｏｌ·Ｌ－１）则能明显降低ＮＯ和ｃＧＭＰ水平。提示：ｄＮＢＰ和ｌＮＢＰ对低糖低氧，ＮＭＤＡ或ＫＣｌ诱导的ＮＯ释放和ｃＧＭＰ生成有相反的作用。
The effects of l3nbutylphthalide(lNBP) and d3nbutylphthalide(dNBP) on extracellular nitric oxide(NO) levels and intracellular cyclic GMP(cGMP) levels were studied in primary cultured rat cortical neuronal cells. Nitric oxide and cGMP levels were measured by using spectrometry and radioimmunological analysis(RIA), respectively. The results showed that dNBP(01～100 μmol·L-1) markedly increased extracellular NO levels and intracellular cGMP levels in primary cultured neurons that were exposed for 10 h to hypoxic/hypoglycemic, NmethylDaspartate (NMDA), or KCl media. On the contrary, lNBP(01～100 μmol·L-1) significantly decreased extracellular NO levels and intracellular cGMP levels. It is suggested that there is a contrary effect of dNBP and lNBP on NO release and cGMP production induced by hypoxia/hypoglycemia, NMDA, or KCl.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第6期418-423,共6页
Acta Pharmaceutica Sinica
基金
国家科委1035工程基金
国家自然科学基金
关键词
丁基苯酞
大脑皮层
脑缺血
一氧化氮
环磷酸鸟苷
3nButylphthalide
Cortical neuronal culture
Nitric oxide
Cyclic GMP
NGnitroLarginine methyl ester
