摘要
报道了含ＳＶ４０复制起点的质粒ＤＮＡ在真核细胞抽提物中进行复制的ＤＮＡ体外复制系统的建立．在外源性蛋白质ＳＶ４０大Ｔ抗原（ＳＶ４０Ｔａｇ）的参与下，穿梭质粒ｐＺ１８９能在猴肾ｖｅｒｏ细胞胞浆抽提物中，利用其中参与体内ＤＮＡ复制所需的蛋白质成分，有效地进行体外ＤＮＡ复制．从而为研究真核细胞ＤＮＡ复制系统的结构与功能提供了简单、有效的模型．
In vitro DNA replication system derived from eukaryotes is capable of replicating exogenous plasmid DNA containing SV40 origin of replication efficiently and accurately. Such an in vitro DNA replication system has been established. In the presence of exogenous SV40 large T antigen, shuttlevector plasmid pZ189 could replicate efficiently in monkey kidney vero cytoplasmic extracts, which provided all the other necessary proteins for DNA replication. This in vitro DNA replication system provides a useful measure for studying the composition and function of eukaryotic DNA replication system.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第3期271-273,共3页
Progress In Biochemistry and Biophysics
关键词
穿梭质粒
真核细胞
DNA
复制系统
shuttlevector, eukaryote, DNA replication system, in vitro, SV40 large T antigen
