摘要
为了使ＥＢ病毒能直接感染人上皮细胞，将ｐＳＧ－ＣＲ２－Ｈｙｇ载体转入永生化人上皮细胞（２９３细胞）中。通过间接免疫荧光法测定发现，转化的细胞表达ＥＢ病毒受体。用ＥＢ病毒感染ＣＲ２－２９３细胞后，２３％的细胞可表达ＥＢ病毒抗原。用ＴＰＡ作用于这些细胞后，表达病毒抗原的细胞数增加。用ＰＣＲ法从ＥＢ病毒感染细胞ＤＮＡ中扩增出ＥＢ病毒ＤＮＡＷ片段。在ＴＰＡ持续作用下，ＥＢ病毒感染细胞的形态特征发生改变。把ＥＢ病毒感染细胞接种在裸鼠皮下，每周注射ＴＰＡ，可诱导细胞在裸鼠体内形成肿瘤。经组织病理检查确诊，肿瘤为低分化上皮细胞癌。杂交试验证明，肿瘤组织细胞中有ＥＢ病毒ＥＢＥＲｓ存在。上述结果表明，ＥＢ病毒在ＴＰＡ协同作用下，可诱导永生化上皮细胞恶性转化。
In order to investigate the role of EB virus in the transformation of human epithelial cells,pSG-CR2-Hyg vector was transfected into immortalized human epithelial cells(293 cells)to express. EBV receptors. EBV was used to infect these CR2-293 cells, in which EBV could replicate and express EBV antigens.EBV W fragments were amplified in EBV-infected CR2-293 cells by PCR,EBV-infected epithelial cells grew in piles with multiple cellular layers.When these epithelial cells were transplanted subcutaneously into nude mice and treated with TPA,poorly differentiated carcinomas were induced.Hybridization test indicated that EBERs of EBV were present in tumor tissues.
出处
《病毒学报》
CAS
CSCD
北大核心
1998年第2期133-138,共6页
Chinese Journal of Virology
关键词
鼻咽癌
EB病毒
上皮细胞
癌变
Epstein-Barr virus,CR2, TPA,Nasopharyngeal carcinoma
