摘要
目的探讨肝细胞癌（HCC）与HLA—DRB1基因多态性及DR抗原表达的关系。方法应用PCR-SSP方法与免疫组化技术对61例吉林地区汉族慢性乙型肝炎，44例乙型肝炎肝硬化病人，62例HCC病人及50名健康献血员作对照，进行了HLA-DRB1等位基因分型比较以及肝细胞癌癌组织、癌旁组织及正常肝组织的HLADR抗原表达进行检测。结果慢性乙型肝炎组HLmDRB1＊1201-3等位基因表达频率为17．21％，明显高于正常对照组8．00％（P=0．0427，RR=2．391）；慢性乙型肝炎组、乙型肝炎肝硬化组与肝癌组HLA-DRBl＊0701等位基因频率分别为11．48％、12．50％、11．29％，明显高于正常对照组2．00％（P=0．0066，RR=6．35；P=0．0046，RR=7．00；P=0．0073，RR=6．236）；HLA—DRB1＊1501—5等位基因表达频率在肝癌组为18．55％，明显高于正常对照组9．00％（P=0．0423，RR=2．303）；正常肝组织、肝癌旁组织肝细胞中HLA-DR抗原表达阴性，而34．2％的肝癌组织肝细胞膜和（或）胞浆中可见HI。A—DR抗原表达，呈灶状分布，表达程度与肿瘤大小、癌周血管浸润及转移无关（P〉0．05），与分化程度有关（P〈0．05）。结论HLA-DRB1＊1201—3、HLA-DRB1＊0701等基因型与吉林地区汉族人群慢性乙型肝炎患病相关联，乙型肝炎肝硬化患病与HLA—DRB1＊0701等位基因型相关联，肝癌患病与HLA—DRB1＊1501—5、HLA-DRB1＊0701等位基因型及DR表达量相关联。
Objective To investigate the association of human leucocyte antigen-DRB1 alleles and expression of HLA-DR antigen with Hepatocellular carcinoma（HCC） in Han population of Jilin area. Methods HLA-DRB1 alleles in 61 patients with chronic hepatitis B, 44 patients with hepatic cirrhosis, 62 patients with HCC, and 50 healthy controls were analyzed by using polymerase chain reaction/sequence specific primer technique. Meanwhile the expression of HLA-DR antigen in HCC tissues, tissues adjacent to HCC and nomal liver tissues was detected with immunohistoehemical method. Results The frequency of HLA-DRB1 ＊ 1201-3 allele in chronic hepatitis B group was higher than that in healthy control group（17.21% vs. 8.00%,P=0. 0427, RR=2. 391）. The frequency of HLA- DRB1 ＊ 0701 allele in chronic hepatitis B group, hepatic cirrhosis group and HCC group was markedly higher than that in healthy control group（11. 48% vs. 2.00%, P=0. 0066, RR=6. 351 12. 50% vs. 2.00%, P=0.0046, RR=7.00; 11.29% vs. 2.00%, P=0.0073, RR=6.236）. The frequency of HLA-DRB1 ＊ 1501-5 allele in HCC group was higher than that in healthy control group（18.55% vs. 9.00%, P=0. 0423, RR=2. 303）. The expression of HLA-DR antigen was negative in hepatocytes of nomal liver tissues and tissues adjacent to HCC, while 34.2% of HCC tissues expressed DR antigen which distributed on the cytomembrane and/or in cytoplasm intensively. Expression of DR antigen in HCC was not associated with tumor size, tumor lymphocyte infiltration and tumor metastasis （P〈0.05） ,but it was associated with histological grade. The rate of HLA-DR antigen expression in well- differenciated cases was higher than that in poorly-differenciated ones （P〈0.05）. Conclusions Alleles of HLA-DRB1 ＊ 1201-3 and HLA-DRB1 ＊ 0701 were closed associated with chronic hepatitis B. HLA DRB1 ＊ 0701 was also closed associated with hepatic cirrhosis. Alleles of HLA-DRB1 ＊ 1501 5, HLA DRB1 ＊ 0701 and expression quantity of HLA-DR antigen were associated with HCC in Han popula tion of
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2009年第5期357-361,共5页
Chinese Journal of Hepatobiliary Surgery
基金
吉林省科技发展计划项目基金资助（NO：20060416-4）
