摘要
目的:建立心可舒胶囊中原儿茶醛的HPLC测定含量方法。方法:色谱柱:phenomenes C_(18)柱(200mm×4.6mm, 5μm),流动相:甲醇-水-冰醋酸水溶液(10:90:0.5),流速:1.0 ml·min^(-1),检测波长:280nm。结果:原儿茶醛在0.80～4.0μg范围内有良好的线性关系(r=0.999 7),平均加样回收率为99.87%(RSD=1.0%)。结论:本法简便、灵敏度高,分离效果好,适用于该制剂的质量控制。
Objective ： To establish a method for determination the content of protocatechuic aldehyde in Xinkeshu Capsule by RP- HPLC. Method： The phenomenes C is column （ 200mm × 4.6mm, 5 μm） was used , the mobile phase was methanol-water- glacial acetic acid（ 10： 90：0.5 ） , the flow rate was 1.0 ml. min-1 , and the detection wavelength was 280nm. Result： The linear range of protocatechuie aldehyde was 0.80 - 4.00μg （ r = 0. 999 7 ）, the average recovery was 99.87% （ RSD = 1.0% ）. Conclusion ： The method is simple, sensitive and accurate,it can be used for the quality control of Xinkeshu Capusule.
出处
《中国药师》
CAS
2009年第6期768-769,共2页
China Pharmacist
