摘要
目的探讨脑创伤后脑内白细胞介素－１β（ＩＬ－１β）、细胞间粘附分子－１（ＩＣＡＭ－１）及血管细胞粘附分子－１（ＶＣＡＭ－１）与继发性脑损伤（ＳＢＩ）发病的关系。方法用原位杂交（ＩＳＨ）、免疫细胞化学（ＩＣＣ）等方法，观察了脑创伤后脑内ＩＬ－１β、ＩＣＡＭ－１及ＶＣＡＭ－１的变化。结果侧脑室注射重组人ＩＬ－１β（ｒｈＩＬ－１β），显著增加多形核粒细胞（ＰＭＮ）在受伤脑组织中的侵入和聚集；而给予ＩＬ－１β抗体和Ｎ－乙酰半胱氨酸（ＮＡＣ），则明显减少ＰＭＮ在受伤脑组织中的侵入和聚集。提示，脑创伤后受伤脑组织、ＩＬ－１β、ＩＣＡＭ－１及ＶＣＡＭ－１的表达分泌增加与ＰＭＮ在受伤脑组织中的侵入和聚集明显相关。结论：脑创伤后脑内炎症反应，包括脑创伤后ＰＭＮ在受伤脑组织内侵入和聚集，可能在ＳＢＩ发病过程中起重要作用。
Aim To evaluate the contribution of Interleukin 1β(IL 1β), Intercellular adhesion molecule 1(ICAM 1) and vascular cell adhesion molecule 1(VCAM 1) to the secondary brain injury (SBI). Methods The changes of IL 1β, ICAM 1 and VCAM 1 in brain after cerebral injury were measured by the methods of in situ hybridization (ISH) and immunocyto chemistry(ICC). The relationship between the secretion and expression of these parameters and the infiltration and accumulation of polymorphonuclear neutrophic(PMN) in the injured brain was also studied. Results Injection of IL 1β in cerebral lateral ventricle increased the infiltration and accumulation of PMN in injured brain, but anti IL 1β and N acceltycystine (NAC) reduced them. It indicated that there was a definite relationship between the increase of expression and secretion of IL 1β, ICAM 1 and VCAM 1, and the infiltration of PMN in the injured brain. Conclusion The inflammatory response, including the infiltration and accumulation of PMN, is related to the increase of IL 1β, ICAM 1 and VCAM 1 after cerebral injury, which may play an important role in SBI.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1998年第2期96-98,共3页
Chinese Journal of Trauma
基金
国家自然科学基金重点项目
关键词
颅脑损伤
白细胞介素1
ICAM-1
VCAM-1
Impacted brain injury Secondary brain injury Interleukin 1β Intercellular adhesion molecule 1 Vascular cell adhesion molecule 1
